消瘀止血片对出血性血小板病患者膜糖蛋白表达的影响  

Effect of Xiaoyu Zhixue Tablets on Expression of Membrane Glucoprotein in the Patient of Hemorrhagic Thrombopathy

作　　者：沈霖[1] 沈迪[1] 高兰[1] 刘仲萍[1] 杨艳萍[1] 谢晶[1] 周丕琪[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院中医科

出　　处：《中医杂志》2005年第4期270-272,共3页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(30171194)

摘　　要：目的:观察消瘀止血片对出血性血小板病患者血小板膜糖蛋白表达的影响,探讨其治疗该病的可能机制。方法:148例出血性血小板病患者随机分为两组,中药组98例用消瘀止血片治疗;西药组50例用安络血及维生素 C、K、P 治疗,两组均治疗6个月。观察比较两组治疗后止血疗效及血小板聚集恢复率,并采用流式细胞术检测两组治疗前后及34例健康人(正常组)的血小板膜糖蛋白 GPⅠb/Ⅸ、GPⅡb/Ⅲa、GPⅠb、GPⅡb、GPⅢa和 p-选择素的表达。结果:中药组止血总有效率为89.8%,西药组为54.0%(P<0.01);中药组血小板聚集功能恢复率为72.4%,西药组仅为4.0%(P<0.01)。两组患者治疗前GPⅠb/Ⅸ、GPⅡb/Ⅲa、GPⅠb、GPⅢa及 p-选择素表达荧光强度均低于正常组(P<0.05);经中药治疗后,患者的血小板膜糖蛋白表达显著增加.与正常组比较差异无显著性(P>0.05),且明显优于西药组(P<0.05)。结论:消瘀止血片在受体蛋白水平上调节血小板膜糖蛋白 GPⅠb/Ⅸ、GPⅡb/Ⅲa、GPⅠb、GPⅢa及 p-选择素的表达可能是其治疗出血性血小板病的疗效机理之一。Objective:To observe effect of Xiaoyu Zhixue Tablets on expression of platelet membrane glucoprotein in the patient of hemorrhagic thrombopathy and probe into the mechanism of the therapy.Methods:148 cases of hemorrhagic thrombopathy were randomly divided into a Chinese drug group(n=98)treated by Xiaoyu Zhixue Tablets,and a Western medicine group(n=50) treated by adrenosem,vitamine C,K,P.They were treated for 6 months.After treatment the therapeutic effect of hemostasis and the recovery rate of platelet aggregation in the two group were observed and analyzed.Before and after treatment expressions of platelet membrane glucoproteins GP Ⅰ b/Ⅸ,GP Ⅱ b/Ⅲa,GP Ⅰ b,GP Ⅱ b,GP Ⅲ a and p-selectin expression were detected with flow cytometry in the two groups and in 34 healthy persons(normal group).Results:The total effecive rate of hemostasis was 89. 8% in the Chinese drug group and 54.0% in the Western medicine group,and the recovery rate of platelet aggegation was 72.4% in the Chinese drug group and 4.0% in the Western medicine group,with significant differences(both P<0.01).Before treatment,GP Ⅰ b/Ⅸ,GP Ⅱ b/Ⅲ a,GP Ⅰ b,GP Ⅲ a and p-selectin expression's fluorescent intensities in the patients of the two groups were lower than those in the healthy persons(P<0.05),and after treatment with Chinese drug,the platelet membrane glucoprotein expression significantly increased and was higher than that in the Western medicine group(P<0.05),but with no significant difference as compared with the normal group(P>0.05).Conclusion:Xiaoyu Zhixue Tablets can up-regulate platelet membrane glucoproteins GP Ⅰ b/Ⅸ,GP Ⅱ b/Ⅲ a,GP Ⅰ b,GP Ⅲ a and p-selectin expression,which is possibly one of the mechanisms for treatment of hemorrhagic thrombopathy.

关 键 词：出血性血小板病 消瘀止血片 蛋白表达 患者 GPⅡb/Ⅲa 血小板膜糖蛋白 p-选择素 流式细胞术检测 血小板聚集 GPⅢa 功能恢复率 西药组 中药组 正常组 可能机制 维生素C 治疗前后 止血疗效 观察比较 总有效率 荧光强度 

分 类 号：R259[医药卫生—中西医结合]