健胎液对小鼠着床期子宫内膜孕激素受体及mRNA的影响  

Effects of Jiantai Liquid on Progesterone Receptor(PR)and PRmRNA in the Endometrium of Mice at Nidation Stage

作　　者：刘艳娟[1] 黄光英[1] 杨明炜[1] 龚萍[1] 陆付耳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合科

出　　处：《中医杂志》2005年第4期300-302,共3页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助(30171193)

摘　　要：目的:研究健胎液对胚泡着床障碍小鼠着床期子宫孕激素受体(PR)的影响。方法:将受孕小鼠随机分为正常组、模型组和中药组,采用米非司酮诱导小鼠胚泡着床障碍,予健胎液进行干预,于妊娠第8天处死小鼠,观察子宫内膜形态学变化,采用免疫组化技术检测子宫 PR 的表达,Western 印迹和 RT-PCR 技术检测子宫 PR 及 PR mRNA 表达。结果:模型组子宫内膜发育不良,中药组子宫内膜的发育与正常组相似;中药组子宫内膜腺体、间质 PR 表达范围和强度均较模型组显著增高(P<0.05),与正常组无显著性差异(P>0.05);中药组 PR 蛋白及基因表达均较模型组显著提高(P<0.05),与正常组无显著性差异(P>0.05)。结论:健胎液通过调节胚泡着床障碍小鼠子宫 PR 及 mRNA 的表达,促进子宫内膜发育,改善胚泡着床。Objective:To study on effect of Jiantai Liquid on progesterone receptor(PR)in the uterus at the nidation stage in the mouse with disturbance of blastocyst nidation.Methods:The gestational mice were randomly divided into a normal group,a model group and a Chinese drug group.Mifepristone was used for inducing disturbance of blastocyst nidation in the mice,which were interfered with Jiantai Liquid.At the eighth week of pregnancy,the mice were killed and morphological changes of the endometrium were observed,and PR expression in the uterus was detected with immunohistochemical method and PR and PRmRNA expressions were detected with Western blot and PT-PCR methods.Results:The endometrium showed maldevelopment in the model group and normal development in the Chinese drug group,similar to the normal group;the gland,the PR expression range and intensity of PR expression in the interstitial substance in the Chinese drug group increased as compared with the model group(P<0.05), and with no significant difference as compared with the normal group(P>0.05);expressions of PR protein and gene in the Chinese drug group were significantly higher than those in the model group(P<0. 05),with no significant difference as compared with the normal group(P>0.05).Conclusion:Jiantai Liquid can promote development of the endometrium and improves blastocyst nidation via regulating expressions of PR and PRmRNA in the uterus in the mice with disturbance of blastocyst nidation.

关 键 词：孕激素受体 健胎液 着床期 小鼠 胚泡着床障碍 Western印迹 子宫内膜发育不良 PCR技术检测 显著性差异 mRNA表达 形态学变化 正常组 中药组 米非司酮 免疫组化 基因表达 PR 模型 

分 类 号：R285.5[医药卫生—中药学]