聚合酶链反应（PCR）技术在恶性疟诊断中的应用  

DIAGNOSIS OF FALCIPARUM MALARIA BY POLYMERASE CHAIN REACTION 

作　　者：詹斌[1] 张龙兴[1] 王聚君[1] 冯晓平[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1994年第1期37-39,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学青年基金

摘　　要：应用聚合酶链反应（ＰＣＲ）检测云南疟疾病人血样５３份，结果均为阳性，可出现一条４９２ｂｐ的特异条带，与镜检符合率达１００％；同时检测间日疟血样８份及上海正常人血样１２份，均为阴性，表明本法具有高敏感性及特异性。Polymerase Chain Reaction(PCR) method using a pair of Plasmodium falciparum specific oligonucleotides as primers was used to detect malaria patient blood samples collected from Yunnan endemic malarious area.The results showed that a DNA fragment of 492 bp was amplified in all 53 microscopically diagnosed P.falciparum samples,but negative in 8 P. vivax samples and 12 normal blood samples.This method seems to have high sensitivity and high specificity.

关 键 词：诊断 恶性疟 聚合酶链反应 

分 类 号：R531.304[医药卫生—内科学]