日本血吸虫可溶性虫卵抗原等电点组分的特性分析(英文)  

CHARACTERIZATION OF ISOELECTRIC POINT(pI) FRACTIONS OF SOLUBLE EGG ANTIGEN OF SCHISTOSOMA JAPONICUM

作　　者：朱荫昌[1] Donald A.Harn John J.Quinn 

机构地区：[1]江苏省寄生虫病防治研究所 [2]哈佛公共卫生学院

出　　处：《中国寄生虫学与寄生虫病杂志》1994年第1期13-19,共7页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：联合国开发署世界银行和世界卫生组织热带病研究和培训特别计划的资助

摘　　要：应用等电聚焦技术将日本血吸虫可溶性虫卵抗原（ＳＥＡ）分为２０个不同等电点（ｐＩ）的组分（等电点范围为１．８６—１１．４０），测定其各组分的体液与细胞免疫反应及体外诱发肉芽肿形成反应。结果表明等电点酸性区的组分（ｐＩ３．７８－５．５４）在日本血吸虫肉芽肿形成中可能起重要的作用；抗体，在感染的早期（５ｗｋ）也与肉芽肿的调节有关。The soluble egg antigen of Schistosoma japonicum was fractionated into 20 individual isoelectric point (pI) fractions with different pIs ranging from 1.86-11.40 by isoelectric focusing (IEF). The fractions were tested for humoral and cellular responses, as well as in vitro granuloma formations.The results indicated that the fractions in the acidic region(pI 3.78-5.54)may play an important role in granuloma elicitation in schistosomiasis japonica,and antibodies are also involved in the regulation of granuloma formation especially at the early stage (5 week postinfection).

关 键 词：血吸虫 虫卵抗原 SEA 等电点 

分 类 号：R383.24[医药卫生—医学寄生虫学]