恶性疟原虫裂殖子与N-乙酰葡萄糖胺之间分子识别的研究  

MOLECULAR RECOGNITION BETWEEN GlcNAc AND PLASMODIUM FALCIPARUM MEROZOITES

作　　者：朱奎[1] 魏尧梅[1] 李建新[1] 陈克明[1] 郑尊[1] 颜永碧[1] 

机构地区：[1]第二军医大学

出　　处：《中国寄生虫学与寄生虫病杂志》1994年第1期20-22,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：用自制并纯化的含Ｎ－乙酰葡萄糖胺（ＧｌｃＮＡｃ）的拟糖蛋白（Ｎｅｏｇｌｙｃｏｐｒｏｔｅｉｎ）对恶性疟原虫ＦＣＣ－１／ＨＮ株的裂殖子进行免疫胶金标记。标记样品按常规电镜要求处理，再经过透射电镜观察，结果显示：裂殖子包被周边弥漫状分布胶金颗粒，当环境中存在游离的ＧｌｃＮＡｃ单糖时，则标记受到抑制。从而直观证实：恶性疟原虫ＦＣＣ－１／ＨＮ株裂殖子对ＧｌｃＮＡｃ有识别作用。本研究还进一步证实：ＧｌｃＮＡｃ的Ｎ－乙酰基并非识别所必需。In this study p aminopheny1 2 acetamido 2 deoxy β D glucopyranoside was bound covalently to bovine serum albumin(BSA)to form neoglycoprotein(GlcNAc BSA).The antigenicity of BSA and the pyranose structure of the sugar was preserved.By using the neoglycoprotein antibody against BSA and staphylococcal protein A gold(SPA gold), Plasmodium falciparum FCC 1/HN merozoites were immunolabelled.The labelled sample was observed under transmission electron microscope(TEM).The TEM pictures showed that colloidal gold pellets were distributed all over the merozoite surface.This is the first report on the direct experimental evidence of molecular recognition between GlcNAc BSA(or GlcNAc)and Plasmodium falciparum merozoites.But free GlcNAc or even GlcN can inhibit the immunolabeleation.The results support the hypothesis that,besides N acetylneuraminic acid,GlcNAc is involved as another recognized site on GPA for Plasmodium falciparum .Thus we further confirmd that N acetyl of GlcNAc is not necessary for the recognition.

关 键 词：疟原虫 乙酰葡萄糖胺 免疫胶金标记 

分 类 号：R382.31[医药卫生—医学寄生虫学]