用固相聚合酶链反应检测恶性疟原虫DNA特异片段  

DETECTION OF SPECIFIC DNA FRAGMENT OF PLASMODIUM FALCIPARUM BY SOLID PHASE PCR

作　　者：于洋[1] 何建文[1] 蔡龙荣[1] 叶芳耘[1] 辛宏[1] 

机构地区：[1]上海第二军医大学长海医院实验诊断科

出　　处：《中国寄生虫学与寄生虫病杂志》1994年第2期122-124,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：本文建立了固相聚合酶链反应（ＰＣＲ）技术检测恶性疟原虫的方法，并对培养的ＦＣＣｌ／ＨＮ株及西双版纳恶性疟患者进行检测，结果表明该法对ＦＣＣｌ／ＨＮ株ＤＮＡ敏感到０．２ｐｇ，相当于１０个疟原虫，对西双版纳疟疾患者检测恶性疟３０例，间日疟４例，证实ＰＣＲ仅特异扩增恶性疟原虫ＤＮＡ。提示固相ＰＣＲ是早期、敏感、特异检测恶性疟原虫的方法。Solid phase PCR for detecting Plasmodium falciparum was established and used to detect cultured FCCl/HN isolate and malaria patients in Yunnan Province.The results revealed that the sensitivity of the method for detecting FCCl/HN isolate DNA was as minimal as 0.2 pg,or about 10 parasites. The specificity of the method was confirmed by discriminating malaria patients infected with different species of Plasmodium .The results suggest that solid phase PCR is a specific and sensitive method for early detection of P.falciparum.

关 键 词：聚合酶链反应 疟原虫 DNA 

分 类 号：R382.31[医药卫生—医学寄生虫学]