间日疟原虫红内期cDNA文库的构建  

CONSTRUCTION OF A cDNA LIBRARY OF THE ERYTHROCYTIC STAGE OF PLASMODIUM VIVAX 

作　　者：邱持平[1] 童小妹[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1994年第2期104-106,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：WHO/TDR主任基金

摘　　要：以现场感染间日疟原虫患者血为材料，提取疟原虫总ＲＮＡ，分离纯化ｍＲＮＡ，在逆转录酶的作用下合成双链ｃＤＮＡ，以λｇｔ１１噬菌体为载体，构建ｃＤＮＡ文库。Plasmodium vivax infected blood samples were collected from patients in the field du ring malaria transmission season. Total RNA of the parasites was extracted by guanidine HCl/cesium chloride centrifugation. mRNA was purified through oligo dT cellulose. Double stranded cDNA were synthesized with AMV reverse transcriptase by Huynhs method.λgt11 phage was used as the vector.A cDNA library of the erythrocytic stage P. vivax was constructed after recombination of DNA and package in vitro.

关 键 词：间日疟原虫 疟原虫 CDNA文库 

分 类 号：R382.31[医药卫生—医学寄生虫学]