聚合酶链反应检测恶性疟原虫的研究  

DETECTION OF PLASMODIUM FALCIPARUM BY POLYMERASE CHAIN REACTION (PCR)

作　　者：詹斌[1] 张龙兴[1] 王聚君[1] 冯晓平[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所

出　　处：《中国寄生虫学与寄生虫病杂志》1994年第2期111-114,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学青年基金

摘　　要：根据编码恶性疟原虫红细胞结合抗原（ＥＢＡ－１７５）的部分ＤＮＡ片段而设计合成寡核苷酸引物并进行多聚酶链反应（ＰＣＲ）以检测体外培养的恶性疟原虫（Ｐ．ｆ．）。扩增产物经琼脂糖凝胶电泳分析，可见扩增出了特异的４９２ｂｐ大小的ＤＮＡ片段，而对间日疟原虫（Ｐ．ｖ．）、食蟹猴疟原虫（Ｐ．ｃ．）、约氏鼠疟原虫（Ｐ．ｙ．）、伯氏鼠疟原虫（Ｐ．ｂ．）及正常人血白细胞ＤＮＡ不能扩增出此片段。本法可检原虫密度下限为２０μｌ血中仅含１０个原虫，具有高敏感性和特异性。A pair of primers were designed and synthesized based on the DNA fragment coded erythrocyte binding antigen (EBA 175) of Plasmodium falciparum ( P.f .) and polymerase chain reaction(PCR) was performed to detect P.f. cultured in vitro .The amplified products were analysed by agarose gel electrophoresis and a specific 492 bp DNA band was showed in P.f. infected blood sample,but no band showed in P.vivax, P.cynomolgi , P.yoelii and P.berghei infected blood samples,nor in normal one.The parasite densities detected by this method could be as minimal as 10 parasites/20μl blood.The PCR technique showed high sensitivity and specificity.

关 键 词：疟原虫 聚合酶链反应 

分 类 号：R382.31[医药卫生—医学寄生虫学]