日本血吸虫3－磷酸甘油醛脱氢酶的分离与纯化  

ISOLATION AND PURIFICATION OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM SCHISTOSOMA JAPONICUM

作　　者：王琴美[1] 王鸣杰[1] 常惠玲[1] 

机构地区：[1]中国预防医学科学院寄生虫病研究所,世界卫生组织疟疾,血吸虫病和丝虫病合作中心

出　　处：《中国寄生虫学与寄生虫病杂志》1994年第4期262-264,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：863高科计划

摘　　要：介绍了一种分离与纯化日本血吸虫３－磷酸甘油醛脱氢酶（ＧＡＰＤＨ）的简易方法。通过超速离心、硫酸铰沉淀、ＤＥＡＥ－纤维素（ＤＥ－５２）柱与ＳＤＳ－ＰＡＧＥ分离、纯化，获得了电泳纯的日本血吸虫３－磷酸甘油醛脱氢酶。日本血吸虫具有丰富的ＧＡＰＤＨ，ＳＤＳ－ＰＡＧＥ显示其分子量为３７ｋＤａ，纯化的日本血吸虫的３－磷酸甘油醛脱氢酶可用于研制日本血吸虫候选疫苗的研究。Tbis paper recommends a simple and easy method to isolate and purify glyceraldehyde-3-phosphate dehydrogenase(GAPDH) from Schistosoma japonicum with electrophoretic puri-ty.The recovery rate of 5 6.1% of glyceraldehyde-3-phosphate dehydrogenase from S.japonicum extract was achieved by ammonium sulfate precipitation,DEAE-cellulose columnchromatography and SDS-PAGE.Using SDS-PAGE,the presence of one major band in thefinal purified GAPDH preparation was demonstrated.This protein had a MW of 37 kDa. The authers discuss the important functions of GAPDH and suggest that this method may beuseful in the research of the protective immunity of S.japonicum GAPDH in the developmentof schistosome vaccine.

关 键 词：血吸虫 纯化 GAPDH 分离 日本血吸虫 

分 类 号：R383.24[医药卫生—医学寄生虫学]