以nit和抗药性为遗传标记研究稻瘟病菌的无性重组  

Study on Asexual Recombination in Magnaporthe grisea by Using Nitrate Non-Utilizing (nit) Mutants and Fungicide-Resistance as Independent Genetic Markers

作　　者：张传清[1] 周明国[1] 

机构地区：[1]南京农业大学农业部病虫监测与治理重点开放实验室,江苏南京210095

出　　处：《中国水稻科学》2005年第3期288-290,共3页Chinese Journal of Rice Science

摘　　要：采用硝酸盐利用缺陷突变体(Nitratenon utilizingmutants,nit)为遗传标记研究了稻瘟病菌对异稻瘟净的抗药性在无性重组过程中的遗传学。在DY23(nit1LR)×A73(nitM S)、A73(nitM S)×F42(nit1MR)和F42(nit1MR)×DY24(nitA LR)3个配对组合中都检测到了无性重组体,重组频率分别为7.31%、14.00%和8.63%。大多数无性重组体都有较好的生长、产分生孢子能力和致病力,表明无性重组体有较高的适合度。这些结果说明由菌丝融合导致的无性重组在稻瘟病菌对异稻瘟净的抗药性群体发展中可能起着重要的作用。Genetics of kitazin P resistance in asexual recombination of Magnaporthe griseawas studied by using nitrate non-utilizing (nit) mutants. Asexual recombinants were detected in all the three pairings, namely DY2-3(nit1-LR)×A7-3(nitM-S),A7-3(nitM-S)×F4-2(nit1-MR), and F4-2(nit1-MR)×DY2-4(nitA-LR). The frequencies of the asexual recombinations were 7.31%, 14.00% and 8.63%, respectively. Most of recombinants had satisfied growth, spore production and pathogenicity ability, indicating that asexual recombinants had high fitness. These results suggested that asexual recombination caused by hyphal fusion might play an important role in the development of fungicide resistant population in M. grisea.

关 键 词：无性重组 稻瘟病菌 遗传标记 抗药性 NIT 异稻瘟净 重组体 配对组合 重组频率 分生孢子 群体发展 菌丝融合 突变体 硝酸盐 遗传学 致病力 适合度 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]