左旋氨氯地平对缺氧心肌细胞乳酸脱氢酶和肌钙蛋白的影响  

Effects of Levo-Anlodipine on Lactate Dehydrogenase and Cardiac Troponin of the Anoxic Myocardial Cells

作　　者：刘遂心[1] 孙明[1] 李彤[1] 

机构地区：[1]中南大学湘雅医院心血管病康复中心,湖南省长沙市410008

出　　处：《中国动脉硬化杂志》2005年第1期34-36,共3页Chinese Journal of Arteriosclerosis

摘　　要：目的　探讨钙离子拮抗剂左旋氨氯地平对缺氧心肌细胞损伤标记物及细胞内游离钙离子浓度的影响。方法　在离体乳鼠心肌细胞培养基础上制备心肌细胞缺氧模型 ,随机分为正常对照组、缺氧损伤组和钙拮抗剂干预缺氧损伤组。分别测定各组心肌培养基中乳酸脱氢酶、肌钙蛋白的含量 ,心肌细胞内游离钙离子的浓度 (荧光探针法 )。结果　缺氧损伤组心肌培养基中乳酸脱氢酶和肌钙蛋白的含量及心肌细胞内游离钙离子浓度较正常对照组明显增加 (P均 <0 .0 1) ;经左旋氨氯地平干预后细胞培养基中乳酸脱氢酶和肌钙蛋白的含量及心肌细胞内游离钙离子浓度较缺氧损伤组明显降低 (P均 <0 .0 1)。结论　左旋氨氯地平对缺氧心肌细胞具有保护作用 。Aim To study the effect of levo-anlodipine on lactate dehydrogenase (LDH) and cardiac troponin of the anoxic myocardial cells. Methods Rat myocardial cells were cultured and randomly divided into normal group, anoxic injured group and levo-anlodipine group. Then the anoxic model was set up. After that, the concentrations of LDH, cardiac troponin Ⅰ (cTnⅠ) and dissociative calcium of myocardial cells (with Fura2/am fluorescent probe) were measured in each group. Results The concentrations of LDH, cTnⅠ and dissociative calcium of anoxic group were obviously higher than those of the control group (P<0.01)and levo-anlodipine can lessen the concentrations of LDH, cTnⅠ and dissociative calcium (P<0.01). Conclusion Levo-anlodipine can protect the anoxic myocardial cells by attenuating the calcium overload.

关 键 词：内科学 左旋氨氯地平保护缺氧心肌细胞 荧光探针法 心肌细胞 肌钙蛋白 乳酸脱氢酶 左旋氨氯地平 

分 类 号：R5[医药卫生—内科学]