姜黄素促进损伤骨骼肌修复  

Curcumin promotes skeletal muscle repair

作　　者：蔡冬青[1] 邓美娟[2] 周白菡[2] 李子安[1] 梁嘉雯[1] 李嘉豪[2] 

机构地区：[1]暨南大学生物医学工程系,广东广州510632 [2]香港中文大学医学院解剖系

出　　处：《中国医院药学杂志》2005年第3期208-211,共4页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金(编号:30340038) 暨南大学引进人才基金(编号:692036);香港政府 RGC Earmarked 基金 (编号:CUHK4273/98M);香港中文大学基金(编号:2040788)

摘　　要：目的:研究姜黄素促进损伤骨骼肌修复的功能。方法:制作小鼠胫骨前肌实验性损伤模型,实验小鼠分别肌注和口服姜黄素,以组织学指标观察姜黄素体内促进损伤骨骼肌的修复功能。采用MTT和流析细胞分析技术观察姜黄素对骨骼肌成肌细胞的作用及可能的信号传导通路。结果:实验的小鼠肌肉伤口区新生肌纤维显著多于对照组。姜黄素无明显促C2C12细胞增殖,但能促进C2C12细胞融合而分化成肌纤维;姜黄素能抑制C2C12细胞NFκB反应因子的转活,而对c -Myc和CRE通路反应因子没有显著影响。大剂量使用姜黄素未见急性毒性反应。结论:姜黄素能通过促进骨骼肌干细胞的分化而促进损伤骨骼肌的修复。OBJECTIVE To investigate the ability of curcumin in skeletal muscle healing.METHODS In both control and experimental mice, a deep longitudinal incision was made in the tibialis anterior muscle of the leg. The mice were then injected interpertioneally or fed with curcumin suspended in oil. Histological examinations were used to observe the healing effects of curcumin in vivo. C_ 2C_ 12 myogenic cell line culture combining with transduction profile analysis system, MTT assay and flow cytometry were applied to detect the effects of curcumin in myogenic cell and the possible pathway in vitro. RESULTS It was found that mice given curcumin produced significantly more new myotubules than control muscles. MTT and flow analyses revealed that curcumin had no significant effects on C_ 2C_ 12 cell proliferation. In contrast, it altered the morphology of C_ 2C_ 12 cells and promoted C_ 2C_ 12 cell fusion. There were significantly more myotubules present in curcumin-treated cultures than control cultures. Transduction profile analysis revealed curcumin had no effects on c-Myc and CRE responsive elements. However, it inhibited the transactivation of the NFκB responsive element in C_ 2C_ 12 cells. IκB-GFP transfection analysis revealed that curcumin inhibited NFκB activation by preventing IκB degradation.CONCLUSION The results suggest that curcumin enhanced muscle repair is probably mediated by inhibition of NFκB transcriptional factor. Moreover, curcumin elicited its effect by promoting myogenic differentiation without eliciting a corresponding increase in cell proliferation. In addition, it was found that high dosage of curcumin did not alter the morphology of the kidney, liver and stomach or induced necrosis in these organs. Our findings suggest that curcumin could be used as a therapeutic drug to promote the skeletal muscle healing.

关 键 词：姜黄素 骨骼肌修复 NFκB通路 

分 类 号：R285.6[医药卫生—中药学]