多年生黑麦草愈伤组织诱导与芽分化的研究  

Callus induction and bud differentiation of Lolium perenne

作　　者：李雪[1] 韩烈保[1] 陈季琴[1] 

机构地区：[1]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083

出　　处：《草业科学》2005年第5期45-49,共5页Pratacultural Science

基　　金：国家转基因植物研究与产业化专项(J2002 B 006);国家863项目(2001AA244082)联合资助

摘　　要：对多年生黑麦草Lolium perenne 的2 个品种特拉华(Delaware)与尤文图斯(Juventus)进行了愈伤组织诱导以及芽分化的研究。结果表明,在种子和成熟胚2种外植体中,成熟胚的出愈率高,是较好的愈伤诱导材料。愈伤诱导培养基采用MS基本培养基,附加激素4 mg/L 2,4 D +0 025 mg/L 6 BA。特拉华和尤文图斯较适宜的芽分化培养基是MS基本培养基分别附加0 4 mg/L ZT+0 3 mg/L NAA和0 2 mg/L ZT+0 5 mg/L NAA。特拉华在愈伤诱导和芽分化两方面均优于尤文图斯。Callus induction and bud differentiation of two varieties of Lolium perenne, Delaware and Juventus, were studied in this paper. Mature embryo showed a higher induction rate than seed, and could be used as an explant for callus induction. The medium for callus induction is a basic MS salt mixed with 4 mg/L 2,4-D +0.025 mg/L 6-BA. The medium suitable for bud differentiation for Delaware and Juventus were a basic MS salt with 4mg/L ZT+0.3 mg/L NAA and 0.2 mg/L ZT+0.5 mg/L NAA, respectively. It was found that Delaware was better than Juventus in both callus induction and bud differentiation.

关 键 词：多年生黑麦草 愈伤诱导 芽分化 

分 类 号：S543.6[农业科学—作物学]