以血清药理学方法研究黄连解毒汤对小鼠的药效动力学  

Pharmacodynamic study on Huanglian Jiedu Decoction in mice by serum pharmacological method

作　　者：宋珏[1] 路通[1] 谢林[1] 王广基[1] 刘晓东[1] 

机构地区：[1]中国药科大学江苏省药物代谢动力学重点实验室,江苏南京210038

出　　处：《中草药》2005年第5期709-713,共5页Chinese Traditional and Herbal Drugs

基　　金：国家中医药管理局基金资助项目(02-032P32);国家"863"项目资助(2003AA2Z347A)

摘　　要：目的以含药血清对小鼠肝匀浆体外生成丙二醛(MDA)的抑制作用为指标,研究黄连解毒汤及单味药水煎剂的药效动力学.方法测定小鼠ig黄连解毒汤及单味药所得含药血清对MDA生成作用的经时过程,并考察黄连解毒汤含药血清、单味药煎剂及小檗碱、黄芩苷抑制MDA生成作用的剂量-效应关系.结果小鼠ig黄连解毒汤、黄连、黄芩、黄连+黄芩后,含药血清均可抑制体外肝匀浆MDA生成,且作用呈剂量依赖性.各药的Emax分别为86%、76%、79%及81%;tmax分别为180、120、5及15 min;其煎剂对MDA生成抑制作用呈剂量依赖性;小檗碱在0.12～10 ng/mL无明显的抑制作用;黄芩苷在1.11～10μg/mL有抑制作用,且作用亦呈剂量依赖性.结论黄连解毒汤体内抗氧化作用强于各单方.黄芩苷可能是黄芩药液和含药血清中的抗氧化成分之一,而小檗碱可能与含药血清抗氧化作用无关;小鼠ig黄连解毒汤及其单味药所得含药血清对MDA生成呈时间和剂量依赖性.Objective To study the pharmacodynamic characeristics of Huanglian Jiedu Decoction (HJD) and its single component decoction, such as Rhizoma Coptidis (RC), Radix Scutellariae (RS), and RC+RS in mice by serum pharmacological experiment in vitro. Methods Different doses of the drug-containing serum obtained at different time after ig HJD, RC, RS, and RC+RS to mice were added into the liver homogenate of mice and the formation of malondialdehyde (MDA) was measured with thiobarbituric acid method in order to observe the inhibitory effects of the drug-containing serum on MDA formation, the time-course, and dose-effect relationship. The effects of decoctions of HJD, RC, RS, RC+RS, berberine, and baicalin were investigated as well. Results The drug-containing serum by ig the decoctions of HJD, RC, RS, and RC+RS to mice had the inhibitory effects on MDA formation in liver homogenate in vitro with E_(max) 86%, 76%, 79%, and 81%, and t_(max) 180, 120, 5, and 15 min, respectively, so did baicalin at 1.11—10 ng/mL. All of them showed dose-dependent relationship, but berberine showed no effect on the formation of MDA at 0.12—10 ng/mL. Conclusion HJD is more effective on in vivo antioxidation than the other single components. Baicalin may act as only one of the antioxidants in the drug-containing serum of HJD as well as in the decoctions. Berberine has nothing to do with the inhibitory effect of the drug-containing serum. The obtained serum after ig HJD and its single components to mice may inhibit MDA formation in a dose- and time-dependent manner.

关 键 词：黄连解毒汤 丙二醛(MDA) 血清药理学 药效动力学 

分 类 号：R285.61[医药卫生—中药学]