高效液相色谱法测定复聪香液中淫羊藿苷的含量  

Content Determination of Icariin in Fucongxiang Liquid by HPLC

作　　者：伍毅[1] 郑国燊[1] 

机构地区：[1]广东暨南大学医学院附属第六医院,江门市529000

出　　处：《中国药房》2005年第9期702-703,共2页China Pharmacy

摘　　要：目的建立以高效液相色谱法测定复聪香液中淫羊藿苷含量的方法。方法色谱柱为VP-ODS,流动相为乙腈-水(30∶70),检测波长为270nm。结果淫羊藿苷检测浓度在2.8～28.0μg/ml范围内线性关系良好(r=1.0000),平均加样回收率为100.03%(RSD=0.54%,n=5)。结论本方法简便、灵敏、准确,可用于复聪香液的质量控制。OBJECTIVE:To establish the content determination method of Icariin in Fucongxiang liquid by HPLC.METHODS:HPLC was performed to determine Icariin on VP-ODS column.The mobile phase was acetonitrile:water(30∶70).Detecting wavelength was270nm.RESULTS:The detected concentration of Icariin was in the good linear range of2.8～28.0μg/ml(r=1.0000),and the average recovery was100.03%(RSD=0.54%,n=5).CONCLUSIONS:The estab-lished method is simple,sensitive,accurate and available for the quality control of Fucongxiang liquid.

关 键 词：高效液相色谱法 复聪香液 淫羊藿苷 含量 

分 类 号：R927.2[医药卫生—药学]