大鼠血管紧张素Ⅱ2型受体基因转染对颈动脉球囊损伤后新生内膜形成的影响  

Effects of adenoviral vector mediated rat AT2R gene transfer on intima proliferation after rat carotid artery injury

作　　者：唐兵[1] 何国祥[1] 李德[1] 黎军[1] 

机构地区：[1]第三军医大学附属西南医院心内科,重庆400038

出　　处：《中国介入心脏病学杂志》2005年第2期108-110,共3页Chinese Journal of Interventional Cardiology

摘　　要：目的　探讨腺病毒载体介导的大鼠血管紧张素Ⅱ2型受体(AT2R)基因转染对大鼠颈动脉球囊损伤后新生内膜形成的影响。方法　应用带有绿色荧光蛋白报告基因的重组腺病毒载体将大鼠AT2R基因转移至颈动脉球囊损伤模型中,2 1d后取材,应用免疫组织化学方法检测AT2R在动脉壁中的表达,图像分析观察其对新生内膜形成的影响。结果　在体大鼠颈动脉腺病毒载体的转染率达4 0 % ,AT2R基因在血管壁的新生内膜、中膜、外膜稳定表达,转染AT2R基因的球囊损伤后颈动脉与绿色荧光蛋白基因对照组相比,血管新生内膜 中膜面积比降低了4 8%。结论　AT2R基因转染可抑制颈动脉球囊损伤后新生内膜的形成,对血管成形术后再狭窄可能有一定的预防作用。Objective To study the effect of rat angiotensin Ⅱ receptor (AT2R) gene transfer mediated by adenoviral vector on neointimal proliferation after rat carotid artery injury. Methods AT2R gene was transducted into the carotid artery by adenoviral vector with GFP after carotid balloon injury. Restenosis models were established in Wistar rats. Immunostaining was performed to examin the expression of AT2R in local arteries. Neointima/media area ratio at day 21 after gene transfer was measured by morphometric analysis. Results The transduction rate of AT2R gene in rat carotid arteries was 40% and the gene expressed constantly in the vessel walls. Neointima/media area ratio of the gene transduction group was significantly reduced (48%) compared with the control group at day 21 after gene transfer. Conclusion The results suggest the possibility that AT2R gene transfer can inhibit proliferation of arterial intima after balloon injury and it is a potential therapeutic approach to prevent neointimal hyperplasia.

关 键 词：血管紧张素Ⅱ2型受体 颈动脉球囊损伤 新生内膜形成 基因转染 绿色荧光蛋白报告基因 血管成形术后再狭窄 免疫组织化学方法 绿色荧光蛋白基因 AT2R基因 腺病毒载体介导 重组腺病毒载体 血管新生内膜 损伤模型 基因转移 

分 类 号：R587.2[医药卫生—内分泌] R977.12[医药卫生—内科学]