丙烯腈对体外培养鼠胚胎肢芽细胞增殖分化的研究  

Effect of Acrylonitrile on Proliferation and Differentiation of Rat Embryo Limb Bud Cell In Vitro

作　　者：端礼荣[1] 王卉放[2] 邢光伟[2] 张志坚[1] 

机构地区：[1]江苏大学医学院基础与临床医学研究中心 [2]江苏大学医学技术学院实验中心,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2005年第2期107-109,112,共4页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省教育厅自然科学基金资助项目(02KZ030004)

摘　　要：目的:研究丙烯腈(acrylonitrile,ACN)对大鼠胚胎肢芽细胞增殖分化的影响。方法:13d的大鼠胚胎脊髓组织,经取材、分离、消化后作原代细胞培养,加入不同浓度的ACN0.01,0.1,1.0,10.0,50.0,100.0,200.0μg/ml,从细胞形态学及细胞计数角度观察细胞生长与分化过程,同时测定蛋白质含量、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性并与对照组进行比较。结果:各组ACN明显抑制胚胎肢芽细胞的增殖和分化,集落形成率明显减少,细胞体积小;其半数分化抑制剂量(ICD50)为29μg/ml,半数存活抑制剂量(ICV50)为42μg/ml,均显示剂量效应关系。结论:ACN能抑制胚胎肢芽细胞增殖和分化,可能与其能抑制蛋白质合成,并引起脂质过氧化有关。Objective: To observe the effect of acrylonitrile (ACN) on Limb Bud Cell of rat Embryo. Methods: The primary Limb Bud Cells of rat embryo cultured 13 days were treated with ACN solution ((0.01,)0.1,1.0,10.0,50.0,100.0,200.0 μg/ml). The concentrations of protein and malondialdehyde(( MDA)) were detected , the activity of superoxide dismutase (SOD)was tested. Results: Acrylonitrile can inhibited differerntiation and proliferation and dong formation of Limb Bud cells rat embryo. The concentration of 50% inhibitions of differentiation(ICD50)was 29 μg/mL. The concentration of 50% inhibitions of cells proliferation(ICV50)was 42 μg/mL. Conclusion: The inhibition of acrylonitrile on Limb Bud cells of rat embryo may caused by inhibition of protein synthetis and inducing lipid peroxidation.[

关 键 词：丙烯腈 胚胎肢芽细胞 细胞增殖 分化 脂质过氧化 

分 类 号：R135.99[医药卫生—劳动卫生] R338.2[医药卫生—公共卫生与预防医学]