固定化青霉素酶反应器流动注射碘量法快速测定青霉素  

DETERMINATION OF PENICILLIN IN BROTH BY FLOW INJECTION ANAL YSIS USING AN IMMOBILIZED PENICILLINASE REACTOR AND IODOMETRIC DETECTION

作　　者：李青山 岑军辉 张嗣良[1] 李凡超 俞俊棠[1] 

机构地区：[1]华东理工大学生化工程研究所

出　　处：《中国抗生素杂志》1994年第5期340-343,共4页Chinese Journal of Antibiotics

摘　　要：整个测量系统由沈阳电影反光镜广制造的ＬＺ－１０００型组合式流动注射分析（ＦＩＡ）仪与固定化青霉素酶反应器组成。测定原理同碘量滴定测量青霉素，实验表明：线性范围在０．０６～０．７５ｍｍｏｌ／Ｌ（取样４０μｌ），线性相关系数０．９９８。重现性：对０．１２、０．７５ｍｍｏｌ／Ｌ浓度的青霉素反复测定８次，平均值分别为０．６９０ｍｖ、３．８３８ｍｖ，标准差分别为０．００６６ｍｖ、０．０１６４ｍｖ，精密度分别０．８２％、０．３４％。测定条件影响：在青霉素浓度低于２·５ｍｍｏｌ／Ｌ时，磷酸盐浓度在０．０５～０．２５ｍｏｌ／Ｌ，ｐＨ在６．０～７．５，测定温度在８℃～３０℃对测定结果几乎没有影响。测定速率可达每小时１２０样。在室温（８～２０℃）下使用，每天测定４００样，使用３０天未见活力下降。４℃下保存近半年未见酶反应器活力下降。An automated assay for the determination of penicillin in broth is presented. The method was based on the classical iodometric penicillin assay which is incorporated in a flow injection analysis(FLA)system.The required hydrolysis was performed on line by using an immobilised penicillinase reactor. Various factoers which influence the response of this system and the performance of the system were descri-bed.Good linear relationship was obtained within the ran9of 0.06～0.75 mmol/L(40μL)penicillin. The correlation coefficient of the calibration curve is 0.998.The speed of determination of this system is high(60～120samples per hour).The enzyme in the reactor is stable for at least 6mo-nths.Recycle life of the reactor is more than 30 days.

关 键 词：流动注射分析 青霉素 青霉素酶 碘量法 

分 类 号：TQ465.1[化学工程—制药化工] TQ460.72[医药卫生—药物分析学]