纳米电极-膜片钳监测单个活囊泡连续释放  

Nanoelectrode-patch Clamp Monitoring of Continual Single Living Vesicle Release

作　　者：吴文展[1] 黄卫华[1] 王玮[1] 王宗礼[1] 程介克[1] 张蓉颖[2] 陈宇[3] 刘杰[2] 郑从义[4] 沈超[4] 

机构地区：[1]武汉大学化学与分子科学学院 [2]华中科技大学生命科学与技术学院 [3]华中科技大学生命科学与技术学院,武汉430074 [4]武汉大学生命科学院

出　　处：《高等学校化学学报》2005年第5期829-831,F010,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20299034;20405012)资助.

摘　　要：Carbon fiber nanoelectrode(tip diameter ca. 100 nm)-patch clamp was firstly applied to real-time monitoring dopamine release from single living vesicles of single Rat pheochromocytoma(PC12) cells with highly temporal and spatial resolution. It is first demonstrated that there are continual vesicle releases of dopamine at the same site in the active release zone, which play a main role in the exocytosis.Carbon fiber nanoelectrode(tip diameter ca. 100 nm)-patch clamp was firstly applied to real-time monitoring dopamine release from single living vesicles of single Rat pheochromocytoma(PC12) cells with highly temporal and spatial resolution. It is first demonstrated that there are continual vesicle releases of dopamine at the same site in the active release zone, which play a main role in the exocytosis.

关 键 词：纳米电极 膜片钳 单囊泡空间分辨 连续囊泡释放 

分 类 号：O657[理学—分析化学]