环氧化合物与丝素蛋白化学交联凝胶的结构  

Structure of Chemical Cross-linked Gel of Epoxide with Silk Fibroin

作　　者：闵思佳[1] 陈芳芳[1] 吴豪翔[2] 

机构地区：[1]浙江大学动物科学学院生物资源科学系,杭州310029 [2]浙江大学环境与资源学院,杭州310029

出　　处：《高等学校化学学报》2005年第5期964-967,F009,共5页Chemical Journal of Chinese Universities

基　　金：浙江省自然科学基金(批准号:399474);杭州市"5112工程专项"(批准号:2003133E39)资助.

摘　　要：对丝素蛋白水溶液与环氧化合物交联剂(PGDE)反应制备的丝素凝胶(CFG)的结构进行了研究.用位相差显微镜观察到CFG具有整体均一的形态结构,而未经PGDE交联的纯丝素凝胶(FG)为颗粒聚集结构.CFG的红外吸收光谱在1104cm-1处出现PGDE分子中烷醚的吸收峰,而没有出现PGDE两端环醚的特征吸收峰,显示PGDE已经开环交联在丝素蛋白上.氨基酸分析结果显示,酪氨酸、组氨酸和赖氨酸的含量与反应前相比明显减少,PGDE在这些位点与丝素蛋白发生了交联反应.固体13CNMR谱观察到酪氨酸羟苯基上的碳发生了化学位移,进一步证实了酪氨酸残基与PGDE发生了化学交联.研究表明,PGDE与丝素蛋白交联形成了分子间交联网络结构,从而使CFG成为较为匀质的凝胶,并有较好的透明度和柔韧性.The structure of cross-linked fibroin gel(CFG) prepared by the reaction of cross-linking (agent) epoxide polyethylene glycol diglycidyl ether(PGDE) with silk fibroin solution was investigated. The results indicate that CFG express the whole homogeneous morphological structure observed by site microscopy phase contrast, while the pure fibroin gel(FG), which hadn′t been cross-linked with PGDE, showed the particle-piled structure. There was an absorption peak of alkyl ether in PGDE appearing at 1 104 cm^(-1) of the FTIR spectrum of CFG, while it didn′t show the characteristic peak of epoxide in both sides of PGDE. It revealed that PGDE has been cross-linked with silk fibroin by opening the rings. The amino acid analysis of CFG can be used to explain that the content of tyrosine, histidine and lysine obviously decreased after the reaction, and PGDE cross-linking occured at these sites. The chemical shift of C in tyrosine was observed in solid state ()^(13)C NMR spectra, so that it further proved the cross-linking PGDE with tyrosine residue. It is supposed that an intermolecular network structure can be formed by the cross-linking between PGDE and tyrosine, histidine and lysine residue of silk fibroin, which make CFG homogeneous and with better transparence and flexibility.

关 键 词：丝素蛋白 凝胶 化学交联 环氧化合物 

分 类 号：O631.1[理学—高分子化学]