肝细胞癌组织CD44v6、MMP-2和VEGF的联合表达及其临床意义  

Expression of CD44v6,MMP-2 and VEGF in hepatocellular carcinoma and their clinical significance

作　　者：张庆光[1] 刘毅华[1] 李功文[1] 赵英恒[2] 

机构地区：[1]广州医学院荔湾医院,广州510170 [2]广州医学院第二附属医院,510260

出　　处：《中国肿瘤临床与康复》2005年第2期106-108,共3页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的探讨肝细胞癌(HCC)患者术后组织切片中CD44v6、MMP2和VEGF三种肿瘤标志物的联合表达及其临床意义。方法应用免疫组化方法,用三种物质的单克隆抗体,以S P染色法检测CD44v6、MMP2和VEGF的表达。结果在有肝外转移的HCC组织中,CD44v6阳性率明显高于无肝外转移组;门静脉癌栓组MMP2阳性率明显高于无癌栓组;而VEGF在肿瘤早期、低分化和术后复发的患者组织中的表达有显著性增高;在有肝外转移的患者中上述2～3种标志物的联合表达阳性率明显增加。结论CD44v6、MMP2和VEGF蛋白在HCC的表达与肿瘤的发生、生长、浸润转移有密切的关系;三种蛋白的阳性表达均可促进HCC生长,易形成肝外转移灶或门静脉癌栓;而它们的联合表达,使HCC更容易发生肝外转移。Objective To analyze the combined expression of CD44v6,MMP-2 and VEGF in hepatocellular carcinoma(HCC)and to elucidate their significance.Methods By immunohistochemistry,monoclonal antibody of CD44v6,MMP-2 and VEGF were used to determine the expression of these three substances according to S-P staining method.Results The positive rate of CD44v6 in patients with metastasis was much higher than that in patients without metastasis;the expression of MMP-2 presented a high rate in patients with portal thrombus formation, showing a significant difference as compared with that in patients without portal thrombus; the expression of VEGF in small size, moderately and poorly differentiated and recurrent tumors showed a statistically significant incresase; the combined expression(2 to 3 above markers)was found significantly increased in patients with metastasis.Conclusions The expression of CD44v6、MMP-2 and VEGF in HCC was closely related to carcinogenesis, development, invasion and metastasis of the tumor. The high expression of all these 3 proteins may promote the tumor development, metastasis to other parts and portal thrombus formation and their combined expression promotes frequent metastasis to other parts of the body.

关 键 词：肝肿瘤 CD44V6 MMP-2 VEGF 

分 类 号：R735.7[医药卫生—肿瘤] R730.45[医药卫生—临床医学]