衍生化液相色谱法测定草甘膦原药中痕量甲醛含量  

Determination of Formaldehyde in Glyphosate Technical by HPLC

作　　者：叶贵标[1] 王金芳[1] 张微[1] 黄宝勇[1] 潘灿平[1] 

机构地区：[1]中国农业大学理学院,北京100094

出　　处：《农药科学与管理》2005年第4期9-11,共3页Pesticide Science and Administration

摘　　要：建立了衍生化高效液相色谱法对草甘膦原药中痕量甲醛含量进行测定的方法。样品中残留甲醛经超声波水浴提取,与2,4-二硝基苯肼衍生反应,生成的2,4-二硝基苯腙,用反相色谱柱进行分离,在342nm紫外波长下检测,外标法定量。该分析方法在2-200mg/L浓度范围呈良好线性,添加回收率在88%-106%之间,相对标准偏差<5%。本文实测样品中甲醛的最小检出浓度为0.5mg/kg。An analytical method for determination of formaldehyde impurity in glyphosate technical by off line derivatization-HPLC was developed.The sample was resolved in acetonitrile-water, and extracted within ultrasonic bath.Formaldehyde was determined by a reversed phase HPLC with C18 column using acetonitrile + water (60 + 40 V/V) as mobile phase with 0.8mL/min flow rate and UV detection at 342 nm.The method was simple, rapid and practical.The method was validated from 2 to 200mg/L with satisfied linear relationship.The recovery rates were between 88% to 106% , with less than 5% of coefficient of variation.The lowest detection limit was 0.5mg/kg.

关 键 词：甲醛 草甘膦 高效液相色谱 衍生化 

分 类 号：TQ450.7[化学工程—农药化工]