Cucumis hytivus染色体组间交换重组及其对雄配子育性的影响  

Genomic Exchange and Rearrangement and Its Effect on Fertility of Male Gametophyte in Cucumis hytivus

作　　者：郭军洋[1] 陈劲枫[1] 

机构地区：[1]作物遗传与种质创新国家重点实验室南京农业大学蔬菜研究所,南京210095

出　　处：《武汉植物学研究》2005年第2期107-110,共4页Journal of Wuhan Botanical Research

基　　金：国家高技术研究发展计划专项经费(2002AA241251;2004AA241120);国家自然科学基金资助项目(30170644);教育部<跨世纪优秀人才培养计划>;教育部科技研究重点项目(01097)资助课题。

摘　　要：采用染色体压片技术对双二倍体种Cucumishytivus染色体组间交换重组及其对雄配子育性的影响进行了细胞学研究。找到了该物种染色体组间交换重组的细胞学证据:包括前期的"8"字形、"十"字形结构和中期环状、链状多价体。研究还发现各种可能导致遗传物质不均衡分离的异常结构如染色体桥、染色体滞后、染色体组分离、微核等。染色体组间广泛的交换和重组导致约93%的多分体及部分异常四分体的形成,多分体中大量的畸形小孢子和四分体中遗传物质不平衡的小孢子因不能正常发育而最终形成败育的雄配子,直接影响到Cucumishytivus育性。Genomic exchange and rearrangement of the amphidiploid species Cucumis hytivus and its effect on fertility of male gametophyte were cytologically studied through making slides.Cytolo-(gical) evidences for genomic exchange and rearrangement were found,such as'8'and'cross'configu-(rations) at prophase,chain and ring multivalents at metaphase Ⅰ,as well as various abnormal chro-(mosome) structure including chromosome bridges,lagging,genomic separation,micronuclei etc,which probably resulted in unequivalent distribution of genetic germplasm.Such an extensive geno-(mic) exchange and rearrangement led to 93% polyads and some abnormal tetrads.Abnormal micro-(spores) in polyads and microspores with unbalance germplasm could not develop into normal male gametophytes,which is responsible for the low fertility of male gametophyte in Cucumis hytivus.

关 键 词：CUCUMIS hytivus 染色体组 交换重组 雄配子 

分 类 号：Q943[生物学—植物学]