结球白菜离体子叶不定芽再生过程中的组织学及生理变化  

Histological and Physiological Changes in the Process of in vitro Shoot Regeneration from Cotyledons of Chinese Cabbage

作　　者：樊明琴[1] 朱月林[1] 朱丽华[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《武汉植物学研究》2005年第2期138-143,共6页Journal of Wuhan Botanical Research

基　　金：教育部留学回国人员科研启动基金资助项目([2001]498)。

摘　　要：以日本引进品种爱知结球白菜(Brassicarapassp.pekinensiscv.AichiHakusai,C1)为试材,对离体子叶不定芽再生过程中的组织学和生理变化进行了研究。结果表明,子叶在离体培养过程中,不定芽发生方式为器官直接发生。在不定芽形成前,可溶性蛋白质含量、POD和SOD的活性均呈上升趋势。随着细胞的脱分化,代谢活动逐渐旺盛,酶活性增强,可溶性蛋白质含量增加,表明不定芽形成过程中形态变化与生理变化紧密相联。培养基中添加AgNO3对酶活性有促进作用,并促进不定芽的分化。Aichi Hakusai (C_1), which was a Chinese cabbage cultivar (Brassica rapa ssp. pekinensis (L.) Olsson) introduced from Japan, was used as plant material in this experiment. Investigations were carried out on histological and physiological changes during the process of in vitro shoot regeneration from cotyledonary explants. Histological observations showed that,(adventitious) shoots were induced through the pathway of direct organogenesis during the process of explants culture. Before adventitious shoots initiated, soluble protein content and activities of POD and SOD showed the trend to increase. As the cells were dedifferentiated, metabolism (acti-)(vity) of cells gradually became stronger; enzymes activity and the soluble protein content were elevated. These results demonstrated that, morphological changes were strongly associated with physiological changes during the process of adventitious shoot induction. Addition of AgNO_3 to the medium increased the activity of enzymes and promoted the differentiation percentage of (adventitious) shoots.

关 键 词：结球白菜 不定芽再生 组织学变化 生理变化 

分 类 号：Q943.1[生物学—植物学] S634.1[农业科学—蔬菜学]