分光光度法测定银杏叶中黄酮的含量  

Spectrophotometric Determination of Total Flavonoids in Ginkgo Leaves

作　　者：邓桂春[1] 王鑫[1] 赵丽艳[1] 高虹[1] 张娜[1] 马进[1] 臧树良[1] 

机构地区：[1]辽宁大学化学科学与工程学院,辽宁沈阳110036

出　　处：《辽宁大学学报（自然科学版）》2005年第2期101-104,共4页Journal of Liaoning University：Natural Sciences Edition

摘　　要：采用分光光度法测定银杏叶和银杏叶提取液中总黄酮含量,并对测定条件、干扰因素进行研究.黄酮含量在8～80μg/mL范围内服从比耳定律,回归方程A=11.5602C+0.0085,相关系数r=0.9999,实验测得银杏叶中黄酮含量为1.47%;直接使用70%乙醇热浸取银杏叶,银杏叶提取液中黄酮浓度为600μg/mL,最大提取率为89.12%.Total flavonoids in Ginkgo leaves and its extractive solution were determined by Spectrophotometry . We studied the conditions of determination and the factors of interference.Regression equation was A=11.560 2C + 0.008 5,correlation coefficient r=0.9999,linear range of obeying Bill law :8～80 μg/mL.Content of total flavonoids in Ginkgo leaves was 1.47%.The leaves immerged simply in the 70% alcohol,their best extractive ratio achieved 89.12%.Concentration of extractive solution was 600 μg/mL.

关 键 词：银杏叶 黄酮 浸提 分光光度法 

分 类 号：O657.3[理学—分析化学]