花生幼胚蛋白质双向电泳的方法与改进  

An Introduction of the Improvement on the Two-dimensional Electrophoresis for Protein from Early Young Embryo of Peanut

作　　者：张君诚[1] 蔡晔[2] 张新文[2] 唐荣华[2] 蔡宁波[2] 庄伟建[1] 

机构地区：[1]福建农林大学作物学院油料研究所,福建福州350002 [2]三明高等专科学校化学与生物工程系,福建三明365004

出　　处：《山西农业大学学报（自然科学版）》2005年第1期38-41,共4页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金资助项目 (30070481); 福建省教育厅科技项目 (JA02256)

摘　　要：在对传统的双向电泳方法进行改进和优化的基础上,探索适合花生早期幼胚蛋白质双向电泳的方法与体系;利用该方法经考染后能稳定得到100～200个分散性较好的蛋白质斑点,分子量多集中在20 000～90 000之间.还对一些技术细节进行讨论和分析.Based on the traditional method of O' Farrell, a improved procedure of two-dimensional gel electrophoresis was introduced.It was proved that the analysis of protein from early young peanut embryo was reliable.With the improved method,100~200 protein spots in good distribution were obtained and molecular weights of the proteins were around the range of 20 000~90 000. The results from it was stable and distinct.Some discussion about the details of procedure was also presented in this paper.

关 键 词：双向电泳 蛋白质 早期幼胚 花生 

分 类 号：Q936[生物学—微生物学] S565.2[农业科学—作物学]