基于离子交换吸附的动态液滴荧光法用于维生素B_2和B_6的快速检测  

Dynamic liquid drop spectrofluorimetry for rapid detection of vitamin B_2 and B_6 based on ion exchange

作　　者：陈泽忠[1] 冯锋[1] 林洁刁[1] 廖雪明[1] 

机构地区：[1]山西大同大学化学系,山西大同037009

出　　处：《生物医学工程与临床》2005年第3期136-139,共4页Biomedical Engineering and Clinical Medicine

基　　金：山西省自然科学基金(20051031);山西省教委高科技开发基金(2003033)资助。

摘　　要：目的发展一种新型的分析方法用于维生素B2和B6的联合检测。方法基于葡聚糖凝胶CM-C25对维生素B6的选择性吸附,采用液滴荧光分析方法对维生素B2和B6混合溶液进行检测。结果维生素B2和B6在浓度分别为10～10000μg/L和10～3000μg/L范围内呈线性关系,检出限分别为5μg/L和6μg/L。该方法用于人工合成样品及复合维生素B药片中维生素B2和B6的检测,获得满意的结果。结论该方法快速、灵敏,可用于制药工业中维生素B2和B6含量的检测。Objective To develop a novel method for simultaneous determination of vitamin B2 and B6. Methods Based on the selective sorption of Sephadex CM-C25 resin for vitamin B6, a renewable drop spectrofluorimetry was used for simultaneous determination of vitamin B2 and B6 in a mixed solution. Results Linear calibration curves were obtained over the range of 10～10 000 μg/L and 10 ～ 3 000 μg/L for vitamin B2 and B6 with determination limits of 5 μg/L and 6 μg/L respectively. This method had been applied to determination of vitamin B2 or B6 in B complex tablets and synthetic mixture with satisfactory results. Conclusion This is a rapid and sensitive method for determination of vitamin B2 or B6 content.Thus it might be used in pharmaceutical industry.

关 键 词：液滴传感器 荧光分光光度法 葡聚糖 维生素B2 维生素B6 药物含量测定 离子交换吸附 

分 类 号：TQ466[化学工程—制药化工]