单纯疱疹病毒I型感染鼠的抗感染机理研究  

ANTI-INFECTIOUS MECHANISM OF MICE INFECTED WITHHERPES SIMPLEX VIRUS-I

作　　者：秦慧莲[1] 方舫[1] 何球藻[1] 

机构地区：[1]上海医科大学免疫学教研室

出　　处：《中国免疫学杂志》1994年第2期71-75,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金

摘　　要：单纯疮疹病毒Ｉ型（ＨＳＶ－Ｉ）属疱疹病毒科，它可引起人和动物的急性感染与持续性感染。国外文献报道，在ＨＳＶ－Ｉ感染的康复过程中，细胞免疫起着关键的作用。国内此类报道甚少。研究结果表明：ＨＳＶ－Ｉ标准株（ｋｏｓ）经自行扩增后，测得病毒毒力３．２×１０￣８ＴＣＩＤ５０／ｍｌ，ＬＤ５０为３．２８．并以１０￣４ＰＦＵ，ＨＳＶ－Ｉ经腹腔感染小鼠，建立感染动物模型。同时，用此病毒体外感染Ｐ８１５细胞，可在细胞表面检出ＨＳＶ－Ｉ病毒抗原。当小鼠感染ＨＳＶ－Ｉ病毒后，在感染初期脾细胞诱生ＩＦＮ的能力迅速上升（感染鼠２５６．０±０．０Ｕ／ｍｌ，正常鼠３７．３±６．０Ｕ／ｍｌ），至２１天时降至正常组水平，这一变化与感染鼠ＮＫ活性动态观察结果相吻合。实验还发现，ＨＳＶ－Ｉ感染鼠脾细胞诱生ＩＬ－２的能力也是增高的。虽然在感染早期有一短暂的下降，这可能与动物在感染初期的应激状态有关。结合体外诱导ＬＡＫ及ＨＳＶ－Ｉ／ＣＴＬ实验结果推测，脾细胞诱生细胞因子能力的增高，为体内产生ＬＡＫ及ＣＴＬ提供了条件。体内转移试验进一步证实ＬＡＫ及ＨＳＶ－Ｉ／ＣＴＬ在体内对感染鼠均有不同程度的保护作用，以过继转移ＨＳＶ－Ｉ／ＣＴＬ的保护作用更强。Herpes Simplex Virtis-I(HSV-I)can cause acute and persistent infection in humans andmice. The current studies examined the anti-viral cellular immunity in mice infected withHSV-I. The results demonstrated that(1)The toxicity of the standard strain(KOS)of HSV-I measured was 3.2×10￣8TCID50 and 3.28 LD50 per ml of the virus。HSV-I antigens can befound on the surface of infected P815 cells,(2)Normal mice spleen cells showed NK activityto HSV-I infected P815 ce1ls. LAK activity could also be induced by culture spleen cells wityIL-2,and was more effective killing HSV-I infected P815 targets.(3)In mice infected withHSV-I,the IFN produced by spleen cells were 6.9 times greater than IFN produced byspleen cells from normal mice. The curve of IFN production correlated with detected NK ac-tivity.(4)The capability of spleen cells from HSV-I infected mice to produce IL-2was in-creased with a yield of 116.0±3. 6U/ml in comparison with that of 50.2±3. 5U/ml fromthe normal mice, (5)Adoptive transfer of LAK cells or HSV-I specific CTL could both pro-tect mice from HSV-I infection,however,a stronger in vivo protection was shown by HSV-Ispecific CTL. It showed that the HSV-I virus antigens not only induced the activity of NKand LAK cells with the capability to induce cytokines,but also induce HSV-I specific CTLwhich performs more important more important role against HSV-I infection.

关 键 词：单纯疱疹病毒 杀伤细胞 过继转移 

分 类 号：R392.12[医药卫生—免疫学]