OKT3＋rhIL－2激活胎儿脾细胞的研究  

OBSERVATION ON THE ACTIVATION OF HUMAN FETAL SPLENIC LYMPHOCYTES BY OKT3 AND rhIL-2

作　　者：张洪涛[1] 王润田[1] 王慧芬 

机构地区：[1]河北医学院基础医学研究所免疫室

出　　处：《中国免疫学杂志》1994年第6期330-333,共4页Chinese Journal of Immunology

摘　　要：观察了１８～２８周龄胎儿脾单个核细胞（ＦＳＭＣ）在体外对ＯＫＴ３＋ｒｈＩＬ－２联合刺激的反应性，结果发现：ＯＫＴ３单独能活化ＦＳＭＣ，最适浓度ＯＫＴ３与ｒｈＩＬ－２联合对ＦＳＭＣ有强协同刺激作用。ＯＫＴ３刺激ＦＳＭＣ后明显促进ＩＬ－２Ｒ表达，表明ＯＫＴ３对ＦＳＭＣ的括化作用与ＩＬ－２／ＩＬ－２Ｒ途径相关。ＯＫＴ３＋ｒｈＩＬ－２协同诱导的ＦＳＭｃ能产生ＮＫ和ＬＡＫ活性，且ＬＭ活住较单用ｒｈＩＬ－２诱导者强，间接免疫荧光染色ＦＡＣＳ分析显示，ＯＫＴ３＋ｒｈｌＬ－２协同激活的ＦＳＭＣ主要是ＣＤ８￣＋Ｔ细胞。结果表明ＦＳＭＣ与成人ＰＢＭＣ－样能被ＯＫＴ３活化。n this study,we reported the responsiveness of fetal splenic mononuclear cells(FSMC)tooKT3 and rhlL-2 The results indicated that OKT3 had a strong mitogenic effect tO FSMC;OKT3 withrhIL-2 had marked synergy. The prmnotion of IL-2R expression by OKT3 suggested that the activation ofFSMC by OKT3 may be related to IL-2/IL-2R pathway。 Both rhIL-2+OKT3 or rhIL-2 alone can induceFSMC to develop LAK and NK activity,The LAK activity were higher in rhIL-2+OKT3 group than inrhIL-2 alone。 The phenotypic analysis showed that rhIL-2+OKT3 mainly stimulated CD8￣+Tcells of FSMC,These resuIts indicated that as adult PBMC,the FSMC were also can be activat-ed by OKT3。

关 键 词：杀伤细胞 细胞毒作用 淋巴细胞 表型 人胎脾 

分 类 号：R392.12[医药卫生—免疫学]