灰斑古毒蛾核型多角体病毒几丁质酶基因及其分子进化  

Chitinase gene of Orgyia ericae single-nucleocapsid nucleopolyhedrovirus (OrerSNPV) and its phylogenetic relationship to other baculovirus ChiA genes

作　　者：聂婷婷[1] 严兴成[1] 许美良[1] 段立清[2] 杨丽荣[1] 张传溪[1] 

机构地区：[1]浙江大学应用昆虫学研究所,浙江杭州310029 [2]内蒙古农业大学农学院,呼和浩特010019

出　　处：《常熟理工学院学报》2005年第2期63-70,共8页Journal of Changshu Institute of Technology

基　　金：国家自然科学基金资助项目(30070032);高等学校全国优秀博士学位论文作者专项资金资助项目(200055)

摘　　要：通过对构建的灰斑古毒蛾Orgyiaericae单粒包埋型核型多角体病毒(OrerSNPV)基因组DNA的EcoRI酶切片段质粒文库的测序分析,在EcoRI—J片段(6.4kb)中鉴定出了编码几丁质酶(ChiA)基因开放阅读框(ORF),chiA基因编码区由1695bp组成,编码564个氨基酸,预计蛋白质分子量为62kD。这也是首次在基因序列水平上研究OrerSNPV。将OrerSNPVChiA氨基酸序列与其它已知的18种杆状病毒ChiA氨基酸序列联配比较,结果表明OrerSNPV与其它杆状病毒ChiA氨基酸有60.3-69.5%同源性,其中与BmNPV、CfDEFNPV和LdMNPV的同源性最高。根据氨基酸序列绘制的分子进化树表明杆状病毒chiA基因至少可以分为SlMNPV、GV和其它NPV三个分支,Or erSNPV与LdMNPV在进化树上关系最为接近。Genomic DNA of OrerSNPV was digested with the restriction enzyme EcoRI and the fragments were cloned into the plasmid pUC19 to build a library for sequencing.An ORF encoding the chitinase_A (ChiA) was identified from the cloned EcoRI—J frament,which was 1695 bp in length and was potential to encode a 564 amino acid polypeptide with predicted molecular weight of 62 kD.This is the first gene reported for OrerSNPV.Alignment analysis showed that OrerSNPV ChiA amino acid sequence shared 60.3-69.5% identities with those of other 18 reported baculovirus ChiAs,and highest identities with those of BmNPV、CfDEFNPV and LdMNPV.A Phylogenetic tree based on the amino acid sequences showed the 19 baculovirus ChiAs could be divided into 3 branches,SlMNPV,GV and other NPVs.OrerSNPV ChiA was most closely related to LdMNPV in the tree.

关 键 词：核型多角体病毒 灰斑古毒蛾 分子进化 几丁质酶基因 氨基酸序列 基因组DNA 蛋白质分子量 杆状病毒 chiA基因 开放阅读框 基因编码区 BMNPV 测序分析 质粒文库 酶切片段 基因序列 同源性 进化树 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治] S433.4[农业科学—植物保护]