猕猴桃实生苗组织培养体系建立的研究  

Establishment of tissue culture system for raising kiwifruit seedlings

作　　者：朱道圩[1] 王静毅[1] 吕宗强[1] 

机构地区：[1]河南农业大学林学园艺学院,郑州450002

出　　处：《落叶果树》2005年第2期5-7,共3页Deciduous Fruits

基　　金：河南省自然科学基金项目(0111011700)

摘　　要：以饱满成熟的猕猴桃种子为起始材料,用 2. 5g·L-1赤霉素处理 5小时,再用次氯酸钠灭菌消毒后,将萌芽的种子接种到MS+蔗糖 20g·L-1 +肌醇 100mg·L-1和 0. 75%琼脂的培养基上,根、茎、叶生长表现良好,初步建立了猕猴桃实生苗组织培养体系。猕猴桃实生苗组织培养同利用茎段、茎尖组织培养一样,培养基中无须附加任何激素。Plump ripe seeds of Actinidia deliciosa used as initial material were treated with gibberellin at 2 5g·L -1 for 5 h,then sterilized with sodium hypochlorite.The germinated seeds were inoculated on the MS medium containing 20g·L -1 sucrose,100 mg·L -1 inosite ,and 0 75%agar.The seeds sent up good shoots with healthy roots and leaves,thus an actinidia seedling tissue culture system has been established.

关 键 词：猕猴桃 实生苗 组织培养 外植体 培养基 

分 类 号：S663.4[农业科学—果树学]