The Selection and Breeding of a Novel Microorganism Strain I and Investigation of Core Model Experiment for MEOR  

作　　者：ZhangZhongzhi WeiXiaofang LuoYijing LiQingzhong LeiGuanglun 

机构地区：[1]Biochemicalengineeringlab,FacultyofChemicalScienceandengineering,ChinaUniversityofPetroleum,Beijing102249,China

出　　处：《Petroleum Science》2005年第2期95-102,共8页石油科学（英文版）

摘　　要：This paper introduces the results of selecting and breeding a micro-organism, Strain I, and its core model experiment investigation for microbial enhanced oil recovery (MEOR). Strain I was separated from the formation water of the Dagang oil field, with analytical results showing that Strain I is a gram-positive bacillus. A further study revealed that this strain has an excellent tolerance of environmental stresses: It can survive in conditions of 70℃, 30 wt% salinity and pH3.5-9.4. Strain I can metabolize biosurfactants that could increase the oil recovery ratio, use crude oil as the single carbon source, and decompose long-chain paraffin with a large molecular weight into short-chain paraffin with a small molecular weight. The core model experiment shows that Strain I enhances oil recovery well. Using 2 vol% of the fermentation solution of Strain I to displace the crude oil in the synthetic plastic bonding core could increase the recovery ratio by 21.6%.This paper introduces the results of selecting and breeding a micro-organism, Strain I, and its core model experiment investigation for microbial enhanced oil recovery (MEOR). Strain I was separated from the formation water of the Dagang oil field, with analytical results showing that Strain I is a gram-positive bacillus. A further study revealed that this strain has an excellent tolerance of environmental stresses: It can survive in conditions of 70℃, 30 wt% salinity and pH3.5-9.4. Strain I can metabolize biosurfactants that could increase the oil recovery ratio, use crude oil as the single carbon source, and decompose long-chain paraffin with a large molecular weight into short-chain paraffin with a small molecular weight. The core model experiment shows that Strain I enhances oil recovery well. Using 2 vol% of the fermentation solution of Strain I to displace the crude oil in the synthetic plastic bonding core could increase the recovery ratio by 21.6%.

关 键 词：BIOSURFACTANT THERMOTOLERANCE salinity tolerance core model flooding experiment microbial enhanced oil recovery. 

分 类 号：TE357[石油与天然气工程—油气田开发工程] TE355.9