颅脑外伤后细胞凋亡与Fas抗原的表达  

Experimental study of apoptosis and Fas antigen expression in craniocerebral trauma

作　　者：马延斌[1] 朱志安[1] 张红[1] 邱建华[1] 肖波[1] 冯东福[1] 

机构地区：[1]上海第二医科大学附属宝钢医院神经外科,201900

出　　处：《中国危重病急救医学》2005年第5期286-288,i002,共4页Chinese Critical Care Medicine

基　　金：上海市属高校自然科学基金资助项目(01B01)

摘　　要：目的探讨颅脑外伤后细胞凋亡与Fas抗原表达的变化。为阐明损伤后神经元丢失的多种途径提供实验依据。方法建立大鼠自由落体脑创伤模型,采用免疫组化技术及细胞透射电镜观察形态学的方法,对伤后挫伤灶旁局部脑组织细胞凋亡和Fas抗原表达进行观察。结果在挫伤灶旁观察到细胞凋亡的发生,神经细胞呈现较为典型的凋亡超微结构变化,Fas蛋白表达在24h达到高峰,随后呈下降趋势。结论颅脑外伤后在局部组织可见细胞凋亡的发生,Fas蛋白表达参与了外伤后神经细胞凋亡的过程。Objective To evaluate the change in Fas antigen expression and apoptosis of neurons, to provide an experimental evidence of loss of neurons in craniocerebral injury, and to provide experimental evidence for clarifying multi - approach of the lost neuron after damage. Methods Brain impact injury was reproduced in SD rat with a free falling impacting device. Using immunohistochemistry method the Fas protein expression was assessed and apoptotic cells were detected with electron microscopy. Results Apoptotic cells were found near the contused area, and Fas - positive cells appeared around the injured and hippocampus areas at about 4 hours, peaked at 24 hours after injury and then reduced in number. Conclusion Apoptosis and necrosis are two forms of cell death in brain tissue following experimental brain contusion. Moreover, the results imply that the Fas - FasL pathway plays a pivotal role in the pathophysiology of post - traumatic neuronal apoptosis.

关 键 词：脑外伤后 FAS抗原表达 透射电镜观察 免疫组化技术 超微结构变化 神经细胞凋亡 蛋白表达 神经元丢失 多种途径 自由落体 方法建立 下降趋势 局部组织 脑创伤 形态学 脑组织 24h 挫伤 

分 类 号：R651.15[医药卫生—外科学]