移植物与受体T细胞的平衡对移植物抗宿主病影响的实验研究  

Experimental study on balance of graft’s and receptor’s T cells in graft-versus-host disease

作　　者：邓晓辉[1] 梁辉[1] 严云[1] 郭若冰[1] 朱杨[1] 马静秋[1] 

机构地区：[1]上海第二医科大学附属新华医院血液科,200092

出　　处：《上海医学》2005年第4期312-314,共3页Shanghai Medical Journal

基　　金：上海市血液研究所胡应洲基金资助项目

摘　　要：目的　建立大鼠异基因骨髓移植模型,受体大鼠按不同的移植物与受体T细胞比例回输供体大鼠骨髓细胞。研究该比例对移植物抗宿主病(GVHD) 的发生及其程度的影响。方法　Wistar大鼠为供鼠,SD大鼠为受鼠,受鼠经全身照射(TBI)+环磷酰胺(CTX)预处理后,分别按不同的移植物与受体T细胞比例(1∶1、2∶1、4∶1)回输供体大鼠骨髓细胞。观察移植后各组受体大鼠生存期及GVHD反应。结果　受体大鼠按4∶1的移植物与受体T细胞比例回输供体大鼠骨髓细胞时,GVHD较严重,而当该比例控制在1∶1 和2∶1时,GVHD程度明显减轻(P<0.05)。结论　移植时将移植物与受体T细胞比例控制在一定范围内,使移植物与受体T细胞保持平衡,既有利于移植物的植入,又可减轻GVHD及其他移植相关并发症的发生。Objective A rat allogeneic bone marrow transplantation model was created with the recipient rats infusing of donor rats’ bone marrow cells at different graft-receptor T cell ratio. The influence of different graft-receptor T cell ratio on the development and the degree of severity of graft-versus-host disease (GVHD) were studied. Methods SD rats were served as the donors and Wistar rats as recipients. The recepients after receiving TBI+CTX regiment were infused donor rats’ bone marrow cells in different graft-receptor T cell ratios (1∶1,2∶1,4∶1), then the survival period and GVHD of each group were observed. Results The degree of GVHD was more severe with this ratio of 4∶1, but milder when it was 1∶1 and 2∶1(P< 0.05). Conclusion During transplantation, the graft-receptor T cell ratio is controlled within certain range, and maintained to keep the balance of grafts and receptor T cells providing a more favorable condition for the transplanted graft, and reducing the occurrence of GVHD with related complications.

关 键 词：移植物抗宿主病 T细胞 受体 实验研究 平衡 Wistar大鼠 GVHD 骨髓细胞 异基因骨髓 相关并发症 移植模型 SD大鼠 环磷酰胺 全身照射 比例 供体 回输 预处理 生存期 移植后 控制 

分 类 号：R457.7[医药卫生—治疗学] R392.12[医药卫生—临床医学]