大鼠全脑照射后海马组织中GFAPmRNA表达的研究  

Expression of GFAP MRNA in Rat Hippocampus after Whole-brain Irradiation

作　　者：杨枢[1] 田野[2] 章国芬[1] 何军[3] 陈列松[2] 

机构地区：[1]苏州大学附属第四医院放疗科,江苏无锡214000 [2]苏州大学附属第二医院放疗科 [3]苏州大学附属第一医院血研所,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2005年第2期178-181,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金 (3 0 170 2 87);江苏省卫生厅资助项目 (K2 0 0 40 6)

摘　　要：目的　通过观察大鼠全脑照射后海马组织中胶质纤维酸性蛋白(glialfibrillaryacidicprotein ,GFAP)mRNA表达的变化,探讨GFAP在放射性脑损伤中的作用。方法　大鼠全脑单次2Gy、10Gy和30Gy照射后1d、30d ,用逆转录聚合酶链技术(RT PCR)观察海马组织中GFAPmRNA的表达变化,并分析照射剂量和照射后时间对其表达的影响。结果　10Gy和30Gy组照后1dGFAPmRNA水平开始升高,分别为正常组的2 .5倍、3倍(P<0 .0 0 1) ,并持续到照后30d ,2Gy组未见明显升高。结论　随着照射剂量的加大和观测时间的延长,大鼠大脑海马内GFAPmRNA表达增高,其终产物GFAP在放射性脑损伤保护和修复机制中有重要作用。Objective To discuss the role of GFAP in brain irradiation injury by observing the expression changes of GFAPmRNA in the hippocampus region of rat after whole-brain irradiation. Methods The model was established in the rat after whole-brain irradiation with the single dose of 4 MeV electron beam. The dynamic expression of GFAPmRNA in the hippocampus was analyzed semiquantitatively at different times(1 and 30 days) and doses(2 Gy10 Gy and 30 Gy) points with RT-PCR. Results The level of GFAPmRNA was elevated significantly 1 day after whole-brain irradiation in 10 Gy and 30 Gy groups (P<0.001),keeping for 30 days, but that was not observed in 2 Gy (P>0.05). Conclusions The up-regulation of GFAPmRNA is time and radiation dose dependent.GFAP plays an important role in protective and imparative mechanism of brain irradiation injury.

关 键 词：脑损伤 辐射 海马 RT-PCR GFAPmRNA 

分 类 号：R811.5[医药卫生—放射医学]