大熊猫细管冻精制备程序的建立与应用  

Development and Application of New Method for in Giant Panda Semen Cryopreservation

作　　者：侯蓉 王基山 张志和 张美佳 兰景超 王成东 余建秋 黄祥明 

机构地区：[1]成都大熊猫繁育研究基地,四川成都610081

出　　处：《中国兽医学报》2005年第3期320-322,共3页Chinese Journal of Veterinary Science

基　　金：成都大熊猫繁育研究基金会资助项目;成都市社发资金资助项目

摘　　要：筛选了不同的冷冻稀释液(TEST,蔗糖-卵黄-甘油液)、不同的冷冻剂型(颗粒法,细管法)、不同甘油终浓度、不同的解冻液(M199,Ham′sF10)等条件,在此基础上,建立了大熊猫细管冻精制备程序。与传统颗粒冻精制备技术相比,本试验极显著地提高了大熊猫冷冻精液质量,冷冻精液活力从35.45%提高到57.88%(P<0.01);顶体完整率从42.25%提高到78.24%(P<0.01)。从1999年至2002年共有14只雌性大熊猫在共计36只次发情配种中采用了细管冻精,较1992～1998年采用传统颗粒冻精的总受胎率提高了11.2个百分点(达到55.6%),其中经产健康适龄雌性大熊猫的受胎率提高了27.3个百分点(达到83.3%),处女大熊猫的受胎率提高了8.8个百分点(达到58.8%)。We studied different factors influencing giant panda semen cryopreservation.These factors include cryodiluent(Test and sucrose-yolk buffer),freezing method(straws and pellets),and final concentration of glycerol,thawing/dilution medium(TCM199 and Ham′s F10).Based on the result,a new semen cryopresevation method has been developed.Comparing with traditional methods of pellets,the quality of the freezing semen remarkably improved,the motility of the thawed semen increased from 35.45% to 57.88%(P<0.01),the intact acrosomes rate elevated from 42.25% to 78.24%(P<0.01).Total 14 females had been applied with these straws for AI from 1999-2002,and the conception rate increased from 44.4% to 55.6%.Especially for those healthy multiparous breeders,the conception rate increased by 27.3% and reached up to 83.3%.Conception rate for maiden panda also increased by 8.8% and reached up to 58.8%.

关 键 词：细管冻精 程序 雌性大熊猫 应用 颗粒冻精 冷冻稀释液 顶体完整率 2002年 1999年 1998年 制备技术 精液质量 精液活力 发情配种 总受胎率 细管法 解冻液 Ham 分点 传统 卵黄 剂型 

分 类 号：S823.34[农业科学—畜牧学] TP31[农业科学—畜牧兽医]