维生素C对紫外线诱发DNA损伤的保护作用  

Protective Effect of Vitamin C on Ultraviolet Radiation-Induced DNA Damage

作　　者：周殿凤 柯惟中 籍康 顾柏平[2] 

机构地区：[1]南京师范大学分析测试中心江苏省光电技术重点实验室,南京210097 [2]南京中医药大学,南京210029

出　　处：《光学学报》2005年第5期643-646,共4页Acta Optica Sinica

摘　　要：用拉曼光谱探讨维生素C对紫外线诱发DNA损伤的保护作用。测量了小牛胸腺DNA水溶液及其添加微量维生素C并经过9min和30min紫外辐射的拉曼光谱,紫外线的辐射照度为18.68W/m2。实验结果表明:在浓度为0.2mmol/L的维生素C存在的条件下,全波段的紫外辐射对DNA只造成了轻微的损伤。DNA被保护可能是因为紫外辐射虽然产生了对DNA损伤很大的自由基,但维生素C具有清除自由基的能力。另外由于DNA和维生素C在260nm处对紫外线都有很强的吸收能力,因此也可能是维生素C对紫外线的吸收能力强于DNA,从而保护了DNA。The protection effect of vitamin C on ultraviolet radiation-induced DNA damage was discussed. The Raman spectra of the mixture of small amount vitamin C and DNA solution with ultraviolet radiation of 9 min and 30 min were measured. The intensity of ultraviolet radiation is 18.68 W/m2. When vitamin C (0.2 mmol/L) was added to DNA solution, ultraviolet radiation only had a slightly influence on the DNA. Though free radicals generated by ultraviolet radiation in the mixture of DNA solution and vitamin C can cause DNA damage, vitamin C has antioxidative property and it can scavenge free radicals and prevent the reaction between DNA and free radicals. In the other way, both vitamin C and DNA have strong absorbancy at 260 nm ultraviolet. Maybe the vitamin C absorbancy of ultraviolet radiation is stronger than DNA's, so DNA was protected.

关 键 词：生物光学 分子光谱学 DNA的损伤保护 拉曼光谱 维生素C 紫外辐射 

分 类 号：O657.37[理学—分析化学]