强激光束边缘对单层皮肤成纤维细胞的光生物调节作用  

Photobiomodulation Action of the Marginal Irradiation of High Intensity KTP Laser Irradiations on Monolayer Skin Fibroblasts

作　　者：黄平[1] 刘承宜[1] 徐晓阳[1] 角建瓴[2] 朱玲[1] 李竹[1] 刘颂豪[3] 

机构地区：[1]华南师范大学激光运动医学实验室,广东广州510631 [2]暨南大学医学院,广东广州510630 [3]华南师范大学信息光电子科技学院,广东广州510631

出　　处：《中国激光》2005年第5期723-728,共6页Chinese Journal of Lasers

基　　金：美国激光医学会2002年度暑期资助项目;国家自然科学基金(60478048;60178003和6027812);广东省自然科学基金重点项目(20011480);一般项目(20031526);激光技术国家实验室开放基金;广东省"千百十工程"优秀人才培养基金(Q02087)资助项目

摘　　要：用单层人类正常皮肤成纤维细胞(HSFs)作为细胞模型,研究高强度激光束边缘低强度激光的光生物调节作用。分别用1064nm和532nm脉冲激光对24组培养的HSFs单层细胞进行照射。研究发现,高脉冲能量的照射导致较低存活率和较大的损伤半径。对接受1064nm照射的HSFs,100mJ照射组和150mJ照射组在照射24h和48h后检测发现显著促进增殖;而对532nm照射的HSFs,100mJ照射组在照射24h和48h后检测发现受到显著抑制增殖。这些结果表明,高强度激光束边缘低强度激光存在光生物调节作用,这可能是影响外科激光的长期效应的重要因素。In this study, cultured monolayer normal human skin fibroblasts (HSFs) were irradiated with the fundamental (1064 nm) and the second harmonics (532 nm) waves of Q-switched Nd:YAG laser beams, to study the photobiomodulation of marginal irradiation of high intensity laser beam. For each laser beam, the power output ranged from 0.2 - 1 W in pulsed mode, and the irradiation time was fixed at 20 s. Cell proliferation was performed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay immediately after laser treatment 24 hours and 48 hours, respectively. It was found that high pulse energy irradiation results in small survival rate and large damage radius, and HSFs proliferation was significantly promoted after 24 hours/48 hours 1064 nm laser treatment with 100 mJ and 150 mJ pulse energy, but significantly inhibited after 24 hours/48 hours 532 nm laser treatment with 100 mJ pulse energy. These results indicate that there is the photobiomodulation for marginal irradiation of high intensity laser beam, which might play an important role in the long term effects of laser surgery.

关 键 词：医用光学与生物技术 光生物调节作用 成纤维细胞 

分 类 号：Q631[生物学—生物物理学] Q253