高效液相色谱法同时测定人血清中卡铂、假尿苷含量  

Determination of carboplatin and pseudouridine in the human serum by HPLC

作　　者：李杰[1] 王景萍[2] 周丽莉[1] 孔永霞[1] 刘书哲[2] 王玲 

机构地区：[1]河南省肿瘤研究所,河南郑州450003 [2]河南省肿瘤医院,河南郑州450003

出　　处：《中国药学杂志》2005年第9期691-693,共3页Chinese Pharmaceutical Journal

摘　　要：目的建立一种同时检测人血清中卡铂、假尿苷含量的高效液相色谱方法。方法色谱条件:Spherigel-C18色谱柱;流动相:含0.5%乙腈的0.02 mol·L-1KH2PO4缓冲液;流速:0.95 mL·min-1;UV:230 nm。结果本法卡铂的线性范围为1-20 mg·L-1,相关系数r=0.990 2,平均回收率96%,最低检测浓度0.5 mg·L-1;假尿苷的线性范围为1-16 mg·L-1,相关系数r= 0.998 6;平均回收率97%,最低检测浓度0.3 mg·L-1。结论本法具有样品处理简单、稳定性高等特点,经临床验证具有较好的实用性且易于推广应用。OBJECTIVE: To establish a method for the determination of carboplatin and pseudouridine in human serum. METHODS: The concentration of carboplatin and pseudouridine was assayed on a Spherigel - C18 column with a mobile phase consisting of acetonitrile-0.02 mol&middotL-1 KH 2PO4(5:1 000) at a flow rate of 0.95 mL&middotmin -1 and detected at 230 nm. RESULTS: The calibration curve of carboplatin was linear over the range of 1-20 mg&middotL-1 (r = 0.990 2). the average recovery of carboplatin was 97%. The limit of determination was 0.5 mg&middotL-1. The calibration curve of pseudouridine was linear over the range of 1-16 mg&middotL-1 (r = 0.998 6). The average recovery of pseudouridine was 97%. The limit of determination was 0.3 mg&middotL-1. CONCLUSION: This method is simple and highly stabile. Clinical application demonstrated that this method was practical.

关 键 词：高效液相色谱法 卡铂 假尿苷 

分 类 号：R917[医药卫生—药物分析学] R969[医药卫生—药学]