SARS病例尸解标本和咽嗽液中病原体检测  

Detection of etiologic agents of autopsy lung of dead cases and pharyngeal gargles of Severe Acute Respiratory Syndrome

作　　者：晏辉钧[1] 赵卫[2] 方丹云[1] 周经姣[1] 张文炳[2] 龙北国[2] 郭辉玉[1] 江丽芳[1] 

机构地区：[1]中山大学基础医学院微生物学教研室,广州510080 [2]南方医科大学热卫系微生物学教研室

出　　处：《中国公共卫生》2005年第5期513-515,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金 (30 340 0 1 3) ;广东省科技攻关项目(532 0 1 4 2 0 2 0 2 8)

摘　　要：目的　确定严重急性呼吸系统综合征(SARS)病例尸解肺组织和咽嗽液中病原体种类。方法　用超薄切片电镜技术观察广东省传染性非典型肺炎流行早期3例死亡病例尸解肺组织标本,用RT- PCR法扩增尸解肺组织和SARS病人咽嗽液中SARS冠状病毒特异核酸片段,并进行序列测定。结果　在死亡病人肺组织中观察到衣原体的网状体、中间体以及原体颗粒,并见衣原体包涵体样结构;用RT- PCR法在2例尸解肺组织扩增出预期大小的DNA片段,测序后经相似性(BLAST)比较,其与SARS冠状病毒相应基因片段高度同源;用巢式RT -PCR技术在SARS病人咽漱液中扩增出SARS冠状病毒特异基因片段。结论　在SARS尸解肺组织中发现衣原体样颗粒,在尸解肺组织和咽漱液中扩增出SARS冠状病毒核酸片段,说明SARS患者可合并衣原体等微生物混合感染。Objective To detect the etiologic agents of severe acute respiratory syndrome(SARS). Methods The autopsy lung of the 3 dead cases of SARS were collected from Guangdong for thin-section electron microscopic examination. Th e gene fragments of SARS-CoV from the autopsy lung and pharyngeal gargles were amplified by reverse transcription-polymerase chain reaction(RT-PCR), and foll owed by sequence analysis. Results Chlamydia-like agent were visualized in all lung tissues of three cases with SARS. Specific gene fragments were amplified from t he lung samples of two cases with SARS, which were comfired by DNA sequencing of Blast to belong to SARS-CoV. Conclusion Chlamydia-like particles were found in EM, an d SARS-CoV could be detected by RT-PCR from the lung samples and pharyngeal ga rgles with SARS.

关 键 词：传染性非典型肺炎 衣原体 SARS冠状病毒 电镜 逆转录多聚酶链式反应 

分 类 号：R373.1[医药卫生—病原生物学]