中药川贝母定量分析方法研究  

Study on analytical method for alkaloids in Bulbus Fritillariae Cirrhosae

作　　者：余华[1] 姜艳[1] 李萍[1] 李绍平[2] 王一涛[2] 

机构地区：[1]中国药科大学现代中药重点实验室,江苏南京210038 [2]澳门大学中华医药研究所

出　　处：《中国中药杂志》2005年第8期572-575,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金 (3 0 0 70 886);教育部跨世纪人才基金 ([2 0 0 2 ] 48号 )

摘　　要：目的:研究常用中药川贝母的定量分析方法。方法:应用高效液相色谱法。以4种川贝母共有的有效成分贝母辛为指标;采用AgilentHypersilBDS C18(4. 0mm×2 5 0mm ,5 μm)色谱柱,以乙腈水 二乙胺(37∶6 3∶0 .0 3)为流动相,流速1 0mL·min-1;Alltech 2 0 0 0蒸发光散射检测器检测,外标两点法定量。结果:贝母辛在0 . 0 3188～1. 0 2 0mg·mL-1呈线性,回收率为10 2. 1% (n =5 ) ,RSD 3. 86 %。根据33批不同产地或来源的川贝母药材中贝母辛的含量结果,确定贝母辛的含量限度为0 . 0 0 70 %。结论:本法为良好的贝母辛的定量分析方法,可用于川贝母药材的质量控制。Objective: To develop a HPLC-ELSD method for the quantitation of peimisine in Bulbus Fritillariae Cirrhosae. Method: HPLC equipped with ELSD was used for the analysis of peimisine in Bulbus Fritillariae Cirrhosae. The separation of peimisine was achieved on a reversed-phase Agilent Hypersil BDS-C (18) column with an isocratic phase system consisting of acetonitrile-water-diethylamine (37∶63∶0. 03) under a flow rate at 1.0 mL·min^(-1). Temperature for the detector drift tube was 115 ℃. The air gas flow rate was 3.0 L·min^(-1). Result: The analysis of peimissine showed good linearity between 0.031 88～1.020 mg·mL^(-1). The average recovery was 102.1%(n=5) with RSD 3.86%. According to the results of the 33 different Bulbus Fritillariae Cirrhosae obtained from various origins, the content limit of peimisine was determined to be 0.007 0%. Conclusion: The assay was successfully utilized to quantify the major biologically active alkaloid in four species of Bulbus Fritillariae Cirrhosae. The developed method is simple, rapid, accurate and sensitive for determining the content of peimisine in crude Bulbus Fritillariae Cirrhosae.

关 键 词：定量分析方法 川贝母 蒸发光散射检测器 高效液相色谱法 AGILENT 贝母辛 外标两点法 常用中药 有效成分 不同产地 含量限度 质量控制 色谱柱 流动相 二乙胺 回收率 RSD 药材 

分 类 号：R284.1[医药卫生—中药学]