HPLC测定沙苑子中沙苑子苷A的含量  

Determination of complanatoside A in Semen Astragali Complanati by HPLC

作　　者：张建军[1] 闫兴丽[1] 张玉杰[1] 王玉萍[2] 张秋菊[1] 曾凤英[1] 李伟[1] 欧丽娜[1] 

机构地区：[1]北京中医药大学,北京100029 [2]中国医学科学院中国协和医科大学药用植物研究所,北京100094

出　　处：《中国中药杂志》2005年第8期600-602,共3页China Journal of Chinese Materia Medica

基　　金：国家"九五"重点科技攻关计划项目 (99 92 9 0 1 0 2 9)

摘　　要：目的:建立沙苑子中沙苑子苷A的含量测定方法。方法:运用高效液相色谱法测定。色谱柱为ZOR BAXEXTEND C18(4. 6mm×2 5 0mm ,5 μm) ,流动相乙腈水 磷酸(2 0∶80∶0 .2 ) ,测定波长2 6 7nm。结果:沙苑子苷A在0 . 0 86～0 . 4 30 μg呈良好的线性关系,加样回收率为99 .8% ,RSD 1. 0 %。结论:本法灵敏、快速、准确,可用于测定沙苑子中沙苑子苷A的含量。Objective: To establish the determination method for complanatoside A in seeds of Astragalus complanatus. Method: An HPLC method has been developed to separate complanatoside A on ZORBAX EXTEND-C (18)(4.6 mm×250 mm, 5 μm) column with acetonitrile-water-phosphoric acid (20∶80∶0.2) as mobile phase and UV detection at 267 nm. Result: The good linearity of complanatoside A ranged 0.086-0.430 μg, r=0.999 9. An average recovery of 99.8% (n=5) was obtained with a RSD of 1.0%. Conclusion: The established method is proved to be stability, fast, accurate and can be used for quantification of Complanatoside A in Semen Astragali Complanati.

关 键 词：沙苑子苷 HPLC测定 高效液相色谱法 含量测定方法 加样回收率 测定波长 线性关系 色谱柱 流动相 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]