干姜超临界CO_2提取物质量控制的研究  

Study on quality standard of extracts from Rhizoma Zingiberis by supercritical fluid extraction

作　　者：孟青[1] 冯毅凡[1] 郭晓玲[1] 陈耕夫[1] 蔡健聪 

机构地区：[1]广东药学院药物研究所,广东广州510224 [2]深圳东方泰格生物医药有限公司,广东深圳518040

出　　处：《中国中药杂志》2005年第10期750-752,共3页China Journal of Chinese Materia Medica

摘　　要：目的:建立干姜超临界CO2提取物的有效部位质量控制的方法。方法∶采用薄层色谱法,以石油醚(60～90℃)醋酸乙酯冰醋酸(8∶3∶0.2)为展开剂,5%香草醛硫酸溶液为显色剂,对本品进行鉴别;并采用紫外双波长分光光度法和高效液相色谱法对本品进行总酚及有效成分6姜酚的含量测定。结果∶总酚在9.668～38.67μg·mL-1呈良好线性关系(r=0.9999),平均回收率为97.7%,RSD1.96%;6姜酚在0.20～2.00μg呈良好线性关系(r=0.9999),平均回收率为97.9%,RSD0.33%。结论∶方法准确,灵敏、专属性强,可作为该原料的全面质量控制标准。Objective: To establish the quality standard of extracts from Rhizoma Zingiberis by supercritical CO_2 fluid extraction. Method: The extracts were identified by TLC. The total phenols and the 6-gingerol were determined by dual-wavelength UV spectrophotometry and HPLC separately. Result: The recovery of total phenols was 97.7% (RSD 2.0%). The linear range of 6-gingerol is 0.20-2.0 μg, the average recovery was 97.7% (RSD 2.0%). Conclusion: The method is convenient for a good resolution and can be used for the quality control of extracts from Rhizoma Zingiberis.

关 键 词：超临界CO2 提取物 干姜 紫外双波长分光光度法 高效液相色谱法 平均回收率 质量控制标准 6-姜酚 线性关系 薄层色谱法 有效部位 醋酸乙酯 硫酸溶液 含量测定 有效成分 展开剂 冰醋酸 石油醚 显色剂 香草醛 RSD 专属性 总酚 

分 类 号：R284.1[医药卫生—中药学]