核仁区嗜银蛋白作为多环芳烃暴露效应标志的研究  

Argyrophil nucleolar organizer regions as biomarker of effect for polycyclic aromatic hydrocarbon exposure

作　　者：刘爱林[1] 李松涛[1] 李芳[1] 钟晓[1] 袁晶[1] 鲁文清[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系,武汉430030

出　　处：《中华预防医学杂志》2005年第3期168-170,共3页Chinese Journal of Preventive Medicine

基　　金：国家973计划资助项目(2002CB512902;2002CB512905)

摘　　要：目的研究焦炉工人外周血T淋巴细胞核仁区嗜银蛋白(AgNOR)是否可作为多环芳烃暴露的效应标志。方法选取52名焦化厂职工(按焦炉逸散物暴露水平分为高、中、低暴露组)和10名非职业多环芳烃暴露人员(对照组)作为研究对象。采集外周血,经培养、制片、银染,用核仁银染面积/细胞核面积(I/S)衡量T淋巴细胞AgNOR相对含量;同时检测尿1羟基芘的水平,作为接触多环芳烃的内暴露标志。结果高、中、低暴露组和对照组工人尿1羟基芘的平均浓度分别为(16.56±2.77)、(7.17±3.05)、(3.30±2.77)和(3.04±1.58)μmol/mol肌酐,高暴露组与低暴露组和对照组比较,差异有统计学意义;高、中、低暴露组和对照组工人T淋巴细胞AgNOR的I/S值分别为0.056±0.010、0.065±0.013、0.067±0.008和0.076±0.007,高暴露组与其他3组比较以及中、低暴露组与对照组比较,差异均有统计学意义。结论职业多环芳烃暴露可导致尿1羟基芘水平上升和外周血T淋巴细胞AgNOR相对含量下降,提示多环芳烃暴露可损伤T细胞免疫功能,AgNOR有可能作为多环芳烃暴露的效应标志。Objective To study whether the argyrophil nucleolar organizer regions (AgNOR) in T lymphocytes of peripheral blood in coke-oven workers can be used as a biomarker of effect for polycyclic aromatic hydrocarbon (PAH) exposure.Methods Fifty-two male coke-oven workers were divided into three groups according to exposure levels of coke oven emissions: high-exposure, middle-exposure and low-exposure workers. Additionally 10 men without occupational PAH exposure were chosen as control group. Peripheral blood T lymphocytes were cultured, spread on slides and stained with silver nitrate. The ratio of AgNOR area vs. nuclear area (I/S) in T lymphocytes was analyzed. Urinary 1-hydroxyrene (1-OHP) was measured as the internal dose of PAH exposure.Results Mean urinary 1-OHP level in high-exposure group (16.56±2.77 μmol/mol Cr) was significantly higher than those in low-exposure group (3.30±2.77 μmol/mol Cr,P<0.001) and control group (3.04±1.58 μmol/mol Cr,P<0.01). The mean I/S of AgNOR in T lymphocytes in high-exposure group (0.056±0.010) was significantly lower than those in middle-exposure group (0.065±0.013,P<0.05), low-exposure group (0.067±0.008,P<0.01) and control group (0.076±0.007,P<0.001). It was also found that I/S of AgNOR were significantly decreased in middle-exposure group and low-exposure group in comparison with control group (P<0.01,P<0.05).Conclusions The occupational exposure to PAH resulted in increase of 1-OHP in urine and decrease of AgNOR in T lymphocytes. PAH exposure might lead to damage T lymphocytes function and AgNOR may be considered as a biomarker of effect for PAH exposure.

关 键 词：核仁区 嗜银蛋白 多环芳烃 暴露效应 T淋巴细胞 免疫功能 焦化厂 职业卫生 

分 类 号：R131[医药卫生—劳动卫生]