烟草疫霉的产孢和接种方法研究  

Research on the methods of producing zoosporangia and inoculation of Phytophthora parasitica var.nicotianae

作　　者：王万能[1] 全学军[1] 肖崇刚[2] 

机构地区：[1]重庆工学院生物工程学院,重庆400050 [2]西南农业大学植物保护学院,重庆400716

出　　处：《植物保护学报》2005年第1期18-22,共5页Journal of Plant Protection

基　　金：重庆市教委科学技术研究项目资助(11807)

摘　　要：烟草疫霉是重要的病原真菌,但在人工培养条件下难以培养、产孢以及释放游动孢子。作者对烟草疫霉培养、产孢、接种方法进行了研究。芝麻培养基、黑麦培养基、燕麦培养基可用来培养烟草疫霉,烟草疫霉在三种培养基上平均每天生长量为1.10、0.78、0.86cm;芝麻培养基、黑麦培养基为烟草疫霉产孢子囊较好的培养基,产孢能力强,分别为2.52×10~4、2.07×10~4个孢子囊/cm^2。而且孢子囊释放率高,分别为43.0%、32.1%。游动孢子囊悬浮液灌根接种和注射接种都能使烟草发病,但不同的接种方法和接种浓度下,病情指数不同。Phytophthora parasitica var.nicotianae is an important pathogenic fungus,but it is difficult to cul- ture and produce zoosporangia on medium.Through experiments,the sesame agar medium,rye agar medium and oat agar medium can be used to culture Phytophthora parasitica var.nicotianae.Their growth speeds were 1.10,0.78 and 0.86 cm per day respectively.The sesame agar and rye agar can be used to produce zoospor- angia and their outputs of zoosporangia were 2.52×10~4,2.07×10~4 zoosporangia/cm^2 respectively,and their Release rates were 43.0% and 32.1% respectively.Tobaccos were inoculated by pouring and injecting differ- ent concentration zoospores suspension,disease indexes were directly related to the concentration and inocula- tion method.

关 键 词：烟草疫霉 接种方法 培养基 孢子囊 病原真菌 培养条件 游动孢子 产孢能力 注射接种 接种浓度 病情指数 生长量 释放率 悬浮液 芝麻 黑麦 燕麦 发病 灌根 

分 类 号：S432.4[农业科学—植物病理学]