土壤拮抗放线菌s-930-6菌株活性产物抑菌作用  

Effect of the metabolite of antifungal soil actinomycete s-930-6 strain

作　　者：孙现超[1] 安德荣[1] 李爱荣[1] 刘翠娟[1] 

机构地区：[1]西北农林科技大学植物保护学院,杨凌712100

出　　处：《植物保护学报》2005年第1期33-36,共4页Journal of Plant Protection

基　　金：科技部企业技术创新基金(100C26216101344)

摘　　要：研究结果表明,用生长速率法测定自秦岭太白山区土壤中分离出的拮抗放线菌 s-930-6菌株,其发酵液粗提物对供试病原菌都有不同程度的抑制作用,其中对小麦根腐病菌的菌丝生长作用最强,EC_(50)为2.6g/L。用孢子萌发法测定,该粗提物对黄瓜枯萎病菌和番茄枯萎病菌的孢子萌发抑制作用较强,EC_(50)分别为0.04、0.02g/L。用 D101大孔树脂对粗提物进行提取得到精提物,它对5种供试病菌的菌丝生长在500mg/L浓度下有不同程度的抑制作用,其中对小麦赤霉病菌、小麦根腐病菌和黄瓜枯萎病菌的抑制效果超过85%。精提物在500mg/L 浓度下对小麦白粉病的保护作用为76.39%,对黄瓜霜霉病保护作用为82.38%,治疗作用为78.31%。用高效液相色谱(HPLC)分离纯化精提物得到4个组分,其中 B 组分对小麦赤霉病菌的抑制作用最强,达到94.14%,初步鉴定 B 组分为氨基糖苷类化合物。The rude abstracts of the fermentation broth of s-930-6 strain isolated from the soil of Qinling Taibai Mountain exhibited strong antifungal activity against the mycelium growth of Bipolaris sorokiniana and the spore germination of F.oxysporum f.sp.cucumbrum Owen and F.oxysporum f.sp.lycopersici.The EC_(50) were 2.6g/L,0.04g/L and 0.02g/L respectively.A refined complex was refined from the rude abstracts by D101 macroreticular resin,which could inhibited the mycelium growth of Fusarium graminearum,Bipolaris sorokiniana and F.oxysporum f.sp.cucumbrum Owen.The inhibition efficiencies were all above 85% at 500mg/L concentration.In the pot tests,the refined complex had protective efficiency of 76.39% against Erysiphe graminis at the concentration of 500mg/L.The protective efficiency against Psudoperonospora cubensis was 82.38% and the therapeutic efficiency was 78.31% at the concentration of 500mg/L.Four compounds were isolated and purified from the refined complex by HPLC.Fraction B with the strongest antifungal activity of 94.14% against Fusarium gramirtearum was identified as aminoglycoside compound by general analysis methods.

关 键 词：放线菌 抑菌作用 高效液相色谱(HPLC) 菌株 拮抗 土壤 黄瓜枯萎病菌 小麦赤霉病菌 抑制作用 产物 活性 根腐病菌 EC50 孢子萌发 保护作用 生长速率法 小麦白粉病 黄瓜霜霉病 氨基糖苷类 粗提物 研究结果 生长作用 大孔树脂 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]