新型除草剂丙酯草醚的作用机理  

Mechanism of action of the novel herbicide ZJ0273

作　　者：陈杰[1] 袁军[1] 刘继东[2] 付群梅[3] 吴军[2] 

机构地区：[1]浙江化工科技集团有限公司,杭州310023 [2]浙江大学,杭州310010 [3]中科院上海有机化学研究所,上海200032

出　　处：《植物保护学报》2005年第1期48-52,共5页Journal of Plant Protection

基　　金：国家"十五"科技攻关项目(2001BA308A02-12)

摘　　要：采用常规的室内生测和生化方法,开展新型除草剂丙酯草醚的作用机理研究。试验结果表明:同时添加5mg/L 浓度的三种支链氨基酸即缬氨酸、亮氨酸和异亮氨酸能完全恢复丙酯草醚对高粱茎的生长抑制作用,而添加相同浓度的单一支链氨基酸只能部分消除其抑制作用。离体条件下,丙酯草醚 IC_(50)>100mg/L,而双草醚 IC_(50)值为10^(-5)～10^(-4)mg/L,说明丙酯草醚对离体 ALS 没有抑制作用;活体条件下,丙酯草醚对 ALS 有一定的抑制作用,且随着处理时间的延长,酶活力降低,对 ALS 的抑制作用增加。因此,丙酯草醚使植物体内必需的支链氨基酸合成受阻,仍然属于 ALS 抑制剂,但其作用方式不同于磺酰脲类和嘧啶水杨酸类除草剂等典型的 ALS 抑制剂,它类似于前体农药,即在植物体内通过代谢活化来发挥作用。The mechanism of the novel herbicide ZJ0273 is studied with the conventional bioassay and bio- chemical methods.The results indicated that the addition of 5 mg/L of 3 branched-amino acids to sorghum seedling cultures incubated in the presence of ZJ0273 completely alleviated herbicide-induced stem growth in- hibition.But each of Val,Ile and Leu single was only partly effective in reversing ZJ0273-induced growth in- hibition at the same concentrations.The IC_(50) values of ZJ0273 and bispyribac-sodium were respectively over 100 mg/L and in the ranges of 10^(-5)-10^(-4)mg/L in vitro,so ZJ0273 showed little inhibition effect on acetolac- rate synthase (ALS).ALS was greatly inhibited by ZJ0273 in vivo,and the activity of ALS was lowered when the treat time increasing.It is concluded that ZJ0273 inhibited biosynthesis of the essential branch amino acids and acted as an ALS inhibitor.However,the mode of action of ZJ0273 differs from the other typically commer- cial ALS inhibitors,such as sulfonylurea and pyrimidine salicylic acid herbicides.Its mechanism is similar to a pre-herbicide,and activated by the metabolic processes in the plants.

关 键 词：丙酯草醚 新型除草剂 作用机理 支链氨基酸 生长抑制作用 ALS抑制剂 植物体内 生化方法 机理研究 试验结果 异亮氨酸 离体条件 IC50 处理时间 水杨酸类 磺酰脲类 作用方式 代谢活化 缬氨酸 双草醚 酶活力 AIS 浓度 添加 

分 类 号：S482.4[农业科学—农药学]