小菜蛾溴氰菊酯抗性和敏感种群遗传差异的cDNA RDA分析  

Genetic analysis of diamondback moth,Plutella xylostella,with different susceptibilities to deltamethrin by cDNA representational difference analysis

作　　者：王淑珍[1] 程罗根[1] 陈之浩[2] 李忠英[2] 

机构地区：[1]南京师范大学生命科学院,南京210097 [2]贵州省农业科学院植物保护研究所,贵阳550006

出　　处：《植物保护学报》2005年第1期63-66,共4页Journal of Plant Protection

基　　金：国家自然科学基金(30060051)

摘　　要：寻找小菜蛾抗溴氰菊酯品系中差异表达基因,初步分析小菜蛾敏感品系与抗溴氰菊酯品系间的遗传差异。采用改进的 cDNA 代表性差异分析法,以小菜蛾抗溴氰菊酯品系的 cDNA为 Driver 扩增子、敏感品系的 cDNA 为 Tester 扩增子,通过四轮消减杂交,获得1条200bp 左右的差异扩增带。将此差异扩增带克隆于 pMD18-T 载体,随机挑选10个克隆测序。将所得的10个序列与 GenBank 等数据库作同源比较,发现仅有1个序列与已知泛素基因有较高同源性,但它与小菜蛾抗药性的关系尚未查明。Differentially expressed sequences between Plutella xylostella susceptible strain and the delta- methrin-resistance strain were studied.The improved cDNA representational difference analysis was performed using cDNA from the P.xylostella deltamethrin-resistance strain as Driver amplicon and cDNA from the sus- ceptible strain as Tester amplicon.After four rounds of subtractive hybridization,one different product with a size of about 200 bp was obtained.The fragment was cloned with pMD18-T vector and 10 clones that picked up by random were sequenced.Comparisons between our sequencing result and data in GenBank showed that there was one sequence appearing relatively high homology to ubiquitin gene,but we can't conform that it was correlated to insecticide resistance at present.

关 键 词：CDNA 溴氰菊酯 遗传差异 小菜蛾 敏感种群 RDA 代表性差异分析法 GenBank 抗性 差异表达基因 Driver 敏感品系 初步分析 消减杂交 克隆测序 同源比较 泛素基因 T载体 数据库 同源性 抗药性 序列 

分 类 号：S433[农业科学—农业昆虫与害虫防治]