生防木霉菌对嘧霉胺抗药性变异及其相关蛋白诱导  

Mutation of Trichoderma in tolerance to pyrimethanil and induction of related protein

作　　者：陈红漫[1] 陈捷[2] 阚国仕[1] 李贺民[1] 

机构地区：[1]沈阳农业大学生物科技学院,沈阳110161 [2]上海交通大学农业与生物学院,上海201101

出　　处：《植物保护学报》2005年第1期77-80,共4页Journal of Plant Protection

基　　金：辽宁省博士启动基金(001045)

摘　　要：选育对杀菌剂具有抗性的生防菌株是生物防治研究的重要内容之一。通过药剂选择培养基及紫外光诱导获得对嘧霉胺具抗性的突变体 KTm-1、KTm-2。研究结果显示,与亲本菌株相比,抗性突变体的耐药性提高20倍以上。抗性菌株在无药培养基上连续转代8次,耐药性未见下降。活体拮抗测定表明,耐药菌株的抑菌能力略高于亲本菌株。PAGE 显示抗性突变体 KTm-1的可溶性蛋白谱带比其亲本菌株多4条,其中 R_f=0.64的蛋白谱带是两个耐药菌株的特异性谱带,可视其为耐药相关蛋白。经 Sephadex G-100柱层析纯化,SDS-PAGE 测定耐药蛋白分子量为44.1KDa。Induce of biocontrol strain tolerance of Trichoderrna to chemical fungicides was studied.Two mu- tants tolerant to pyrimethanil were obtained by UV-light induction on PDA amended with pyrimethaniol from a wild Trichoderma strain.Tolerance level was over 20 times in comparison with that of the mother strain.The mutants could maintain their tolerance after 8 times of transfers on the fungicide free PDA.The soluble protein profile bands of tolerant strains were higher than that of their original sensitive strains among which,the toler- ant strains owned the soluble protein profile band of R_f=0.64.But it was not shown in sensitive strains,and it is called related tolerant protein.This protein was purified from KTm-1 by Sephadex G-100 gel filtration. The molecular weight of tolerant protein was determined to be 44.1 KDa.Two tolerant strains kept the ability of antagonism against Botrytis cinerea in vitro and vivo.

关 键 词：相关蛋白 嘧霉胺 抗药性 木霉菌 SEPHADEX SDS-PAGE 抗性突变体 变异 亲本菌株 耐药菌株 蛋白谱带 紫外光诱导 选择培养基 防治研究 生防菌株 研究结果 抗性菌株 层析纯化 耐药性 杀菌剂 可溶性 特异性 分子量 显示 测定 

分 类 号：S476[农业科学—农业昆虫与害虫防治]