银杏内酯对血小板活化因子加重离体豚鼠心肌缺血再灌注损伤的影响  

Effects of ginkgolides on isolated guinea-pig myocardial ischemia-reperfusion damage exacerbated by platelet activating factor

作　　者：王秋娟[1] 高建[2] 

机构地区：[1]中国药科大学生理教研室,南京210009 [2]首都医科大学药理教研室,北京100054

出　　处：《中国新药杂志》2005年第4期423-427,共5页Chinese Journal of New Drugs

基　　金：国家新药基金(93-67-N-45);江苏省"三药"重点攻关项目(苏科技(2000)357号)

摘　　要：目的:研究银杏内酯对外源性血小板活化因子(PAF)加重离体豚鼠心肌缺血再灌注损伤的影响。方法:采用离体豚鼠心脏Langendorff灌流装置,观察银杏内酯(4,8,16mg·L_(-1))对离体豚鼠心肌再灌注心律失常、心肌组织丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)、三磷酸腺苷酸酶(ATPase)以及冠脉流量(CF)的影响。结果:银杏内酯、普萘洛尔有效减少损伤心肌MDA含量及Ca^(2+)-ATP酶活力,增加GSH-PX活力,并且对抗PAF加重的离体豚鼠心肌缺血再灌注心律失常的发生。结论:银杏内酯对抗PAF加重离体豚鼠心肌缺血再灌注损伤的作用与拮抗PAF受体、抑制自由基产生、减少心肌细胞钙超载、防止心律失常的发生有关。Objective:To investigate effects and mechanism of ginkgolides in the protection of myocardial ischemia-reperfusion(MI/R)damage in vitro. Methods: A experimental model of the isolat- ed guinea-pig MI/R damage exacerbated by platelet-activating factor (PAF) was set up to determine if ginkgolides (4,8 and 16mg·L^(-1)) effected activities of glutathione peroxidase (GSH-PX) and ATPase,and the volume of malondialdehyde (MDA) and coronary flow (CF) in myocardial tissue. Results: Ginkgolides significantly reduced the level of MDA in myocardial tissues, increased activities of GSH-PX and Na^+, K^+-ATPase, and inhibited activity of Ca^(2+)-ATPase. Ginkgolides moderately improved the volume of CF in the MI/R damage exacerbated by PAF. Conclusion: Ginkgolides protects occurrence of arrhythmia during the MI/R by antagonizing activities of PAF and generation of free radicals to reduce the overload of calcium in myocardial cells.

关 键 词：银杏内酯 血小板活化因子 心肌缺血再灌注 

分 类 号：R285.5[医药卫生—中药学] R542.2[医药卫生—中医学]